Kiss Arnold M, Jády Beáta E, Darzacq Xavier, Verheggen Céline, Bertrand Edouard, Kiss Tamás
Laboratoire de Biologie Moléculaire Eucaryote du CNRS, Université Paul Sabatier, 118 route de Narbonne, 31062 Toulouse Cedex, France.
Nucleic Acids Res. 2002 Nov 1;30(21):4643-9. doi: 10.1093/nar/gkf592.
Site-specific post-transcriptional conversion of uridines to pseudouridine in ribosomal RNAs and small nuclear RNAs (snRNAs) is directed by guide RNAs which possess the conserved box H and ACA sequence elements and fold into the consensus 'hairpin-hinge-hairpin-tail' secondary structure. Here, we describe an unusual mammalian pseudouridylation guide RNA, called U93, that is composed of two tandemly arranged box H/ACA RNA domains. The U93 RNA therefore carries two H and two ACA box motifs, all of which are essential for accumulation of the full-length RNA. The human U93 RNA accumulates in Cajal (coiled) bodies and it is predicted to function in pseudouridylation of the U2 spliceosomal snRNA. Our results lend further support to the notion that modification of the RNA polymerase II-transcribed spliceosomal snRNAs takes place in Cajal bodies.
核糖体RNA和小核RNA（snRNA）中尿苷向假尿苷的位点特异性转录后转化由引导RNA指导，这些引导RNA具有保守的盒H和ACA序列元件，并折叠成共有“发夹-铰链-发夹-尾”二级结构。在此，我们描述了一种不寻常的哺乳动物假尿苷化引导RNA，称为U93，它由两个串联排列的盒H/ACA RNA结构域组成。因此，U93 RNA携带两个H盒基序和两个ACA盒基序，所有这些对于全长RNA的积累都是必不可少的。人U93 RNA在卡哈尔（卷曲）体中积累，预计其在U2剪接体snRNA的假尿苷化中发挥作用。我们的结果进一步支持了RNA聚合酶II转录的剪接体snRNA的修饰发生在卡哈尔体中的观点。
