Crowe Mark L, Rana Debashis, Fraser Fiona, Bancroft Ian, Trick Martin
John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK.
Nucleic Acids Res. 2002 Nov 1;30(21):e118. doi: 10.1093/nar/gnf117.
We have developed software that allows the prediction of the genomic location of a bacterial artificial chromosome (BAC) clone, or other large genomic clone, based on a simple restriction digest of the BAC. The mapping is performed by comparing the experimentally derived restriction digest of the BAC DNA with a virtual restriction digest of the whole genome sequence. Our trials indicate that this program identified the genomic regions represented by BAC clones with a degree of accuracy comparable to that of end-sequencing, but at considerably less cost. Although the program has been developed principally for use with Arabidopsis BACs, it should align large insert genomic clones to any fully sequenced genome.
我们开发了一款软件，该软件能够基于细菌人工染色体（BAC）克隆或其他大型基因组克隆的简单限制性酶切，预测其基因组位置。通过将实验获得的BAC DNA限制性酶切结果与全基因组序列的虚拟限制性酶切结果进行比较来进行图谱绘制。我们的试验表明，该程序识别BAC克隆所代表的基因组区域的准确度与末端测序相当，但成本要低得多。尽管该程序主要是为拟南芥BAC而开发的，但它应该能够将大型插入基因组克隆与任何已完成全测序的基因组进行比对。
