Yokosawa A, Arai H, Sato H, Endo M, Motomiya M
Tohoku J Exp Med. 1975 Feb;115(2):129-35.
Glycosaminoglycans from a case of pulmonary fibrosis (desquamative interstitial pneumonia of usual type) was analyzed by means of proteolytic digestion, solvent fractionation, column chromatography on anion exchanger, electrophoresis and enzymatic digestion. The result suggested an increase in dermatan sulfate and possibly of heparan sulfate in the fibrotic lung as compared with the normal. It is preferable to take the samples for analysis from a freshly excised specimen of the lung, because there was a sign of degradation of glycosaminoglycan due to storage of the specimen in a solution of formalin.
通过蛋白水解消化、溶剂分级分离、阴离子交换剂柱色谱法、电泳和酶消化等方法，对一例肺纤维化（普通型脱屑性间质性肺炎）病例的糖胺聚糖进行了分析。结果表明，与正常肺相比，纤维化肺中硫酸皮肤素增加，硫酸乙酰肝素可能也增加。最好从新鲜切除的肺标本中采集样本进行分析，因为标本保存在福尔马林溶液中会出现糖胺聚糖降解的迹象。
Zotero 插件