Zhou Minghui, Dong Mingmin, Wu Yuying, Wang Liang, Yue Baohong
Department of Otorhinolaryngology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou, 450052, China.
Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2006 Mar;20(5):227-30.
To study the expression and significance of Eotaxin gene in nasal mucosa and CCR3 in bone marrow in model of allergic rhinitis.
Twenty SD rats were randomly divided into allergic rhinitis (AR) group and normal control group. Ovalbumin (OVA) sensitized and challenged rats were used as the model of allergic rhinitis. The leucocytes in the smears of bone marrow and peripheral blood were counted, and the expression of CCR3 in bone marrow was detected by immunohistochemistry. The pathologic samples of nasal mucosa were stained with HE. The expression of Eotaxin mRNA in nasal mucosa was detected by in situ hybridization (ISH), and the expression of Eotaxin in nasal mucosa was detected by immunohistochemistry.
The ratio of eosinophils to white cells in bone marrow smears of AR group was significantly higher than that of normal control group (P < 0.01). The ratio of eosinophils to white cells in peripheral bloods mears of AR group was significantly higher than that of normal control group (P < 0.01). Eotaxin positive cell and the expression of Eotaxin mRNA in nasal mucosa of AR group were significantly higher than that of normal control group (P < 0.01). The expression of Eotaxin mRNA significantly positively correlated with the expression of Eotaxin (r = 0.804, P < 0.01). The number of Eotaxin positive cells in nasal mucosa was significantly positively correlated with the number of eosinophils in nasal mucosa of AR group (r = 0.795, P < 0.01). The ratio of CCR3 positive cells to white cells in bone marrow smears of AR group was significantly higher than that of normal control group (P < 0.01). The ratio of CCR3 positive cells to white cells was significantly positively correlated with the ratio of eosinophils to white cells in bone marrow smears of AR group (r = 0.736, P < 0.05).
The expression of Eotaxin and Eotaxin mRNA in nasal mucosa increased, and the expression of CCR3 in bone marrow increased in the model of allergic rhinitis. It supplies a cooperation between CCR3 and Eotaxin to allow rapid mobilization of a pool of bone marrow eosinophils followed by recruitment to the nasal mucosa.
研究嗜酸性粒细胞趋化因子（Eotaxin）基因在变应性鼻炎模型鼻黏膜中的表达及意义，以及骨髓中CC趋化因子受体3（CCR3）的表达及意义。
将20只SD大鼠随机分为变应性鼻炎（AR）组和正常对照组。采用卵清蛋白（OVA）致敏和激发大鼠建立变应性鼻炎模型。计数骨髓涂片及外周血涂片中的白细胞，采用免疫组织化学法检测骨髓中CCR3的表达。鼻黏膜病理标本行苏木精-伊红（HE）染色。采用原位杂交（ISH）法检测鼻黏膜中Eotaxin mRNA的表达，采用免疫组织化学法检测鼻黏膜中Eotaxin的表达。
AR组骨髓涂片嗜酸性粒细胞与白细胞的比值显著高于正常对照组（P<0.01）。AR组外周血涂片嗜酸性粒细胞与白细胞的比值显著高于正常对照组（P<0.01）。AR组鼻黏膜中Eotaxin阳性细胞及Eotaxin mRNA的表达显著高于正常对照组（P<0.01）。Eotaxin mRNA的表达与Eotaxin的表达呈显著正相关（r=0.804，P<0.01）。AR组鼻黏膜中Eotaxin阳性细胞数与鼻黏膜嗜酸性粒细胞数呈显著正相关（r=0.795，P<0.01）。AR组骨髓涂片CCR3阳性细胞与白细胞的比值显著高于正常对照组（P<;0.01）。AR组骨髓涂片CCR3阳性细胞与白细胞的比值与嗜酸性粒细胞与白细胞的比值呈显著正相关（r=0.736，P<0.05）。
变应性鼻炎模型中鼻黏膜Eotaxin及Eotaxin mRNA表达增加，骨髓中CCR3表达增加。这为CCR3与Eotaxin之间的协同作用提供了依据，使骨髓嗜酸性粒细胞池能够快速动员，随后募集至鼻黏膜。
