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[一氧化氮合酶在鸡前庭系统中的定位]

[Localization of nitric oxide synthase in the chicken vestibular system].

作者信息

Nie Guohui, Wang Jibao

机构信息

Department of Otolaryngology, Red Cross Hospital, Shenzhen 518035.

出版信息

Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2002 Aug;16(8):426-7.

Abstract

OBJECTIVE

To locate nitric oxide synthase (NOS) in the chicken vestibular system.

METHOD

The frozen section were processed for NADPH-d histochemistry in a solution containing NADPH and nitroblue tetnazolium (NBT) to demonstrate NOS positive reactivity.

RESULT

NOS positive staining, black-blue in color, was seen at the nerve ending, nerve fibers of the utricul and saculla and ampiculium. Ganglion cells had different activity. The shape of the cells seems to be round or oral.

CONCLUSION

Collectively, data indicate the presence of active NOS in these tissue and suggest modulation of vestibular neurotransmission by nitric oxide.

摘要

目的

在鸡的前庭系统中定位一氧化氮合酶(NOS)。

方法

将冰冻切片置于含有烟酰胺腺嘌呤二核苷酸磷酸(NADPH)和氮蓝四唑(NBT)的溶液中进行NADPH-d组织化学处理,以显示NOS阳性反应。

结果

在椭圆囊、球囊和壶腹的神经末梢、神经纤维处可见呈黑蓝色的NOS阳性染色。神经节细胞具有不同的活性。细胞形状似乎为圆形或椭圆形。

结论

总体而言,数据表明这些组织中存在活性NOS,并提示一氧化氮对前庭神经传递有调节作用。

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