Coman B J
Aust Vet J. 1975 Dec;51(12):560-5. doi: 10.1111/j.1751-0813.1975.tb09381.x.
This study reports on the long-term survival of Taenia pisiformis eggs held under controlled laboratory conditions and in the external environment. Egg viability was assessed by feeding the ova to susceptible rabbits. In the laboratory experiment dried eggs were stored under 4 different temperature and relative humidity regimes. At various time intervals eggs were removed from these environments and fed to rabbits which were subsequently killed and the number of viable cysticerci within them counted. The results show that a high temperature (38 degrees C) and to a lesser extent, a low humidity (33%) are quickly lethal to eggs and few survive for more than 7 days. However, at 4 degrees C and a relative humidity of 90%, some eggs were still viable after 300 days. At the lower temperature there was a noticeable interaction between temperature and relative humidity. For the field study a small area of pasture was enclosed by a rabbit-proof fence and hessian screen. The pasture was first shown to be substantially free of extraneous contamination with T. pisiformis ova and was then artificially seeded with a large number of eggs. Fresh groups of susceptible rabbits were grazed on this pasture at intervals thereafter. The results, expressed as the mean number of cysticerci recovered from rabbits, show that eggs remain viable for several months during winter and early spring, but in summer no viable eggs could be demonstrated after a period of 2 weeks.
本研究报告了在可控实验室条件及外部环境下保存的豆状带绦虫卵的长期存活情况。通过将虫卵喂给易感兔来评估卵的活力。在实验室实验中，将干燥的虫卵储存在4种不同的温度和相对湿度条件下。在不同时间间隔从这些环境中取出虫卵并喂给兔子，随后处死兔子并计算其体内活囊尾蚴的数量。结果表明，高温（38摄氏度）以及在较小程度上低湿度（33%）会迅速杀死虫卵，很少有虫卵能存活超过7天。然而，在4摄氏度和相对湿度90%的条件下，一些虫卵在300天后仍有活力。在较低温度下，温度和相对湿度之间存在明显的相互作用。对于野外研究，用防兔围栏和粗麻布屏障围住一小块牧场。首先证明该牧场基本没有豆状带绦虫卵的外来污染，然后人工播撒大量虫卵。此后每隔一段时间将新的易感兔群放到这块牧场上放牧。以从兔子体内回收的囊尾蚴平均数量表示的结果表明，虫卵在冬季和早春的几个月内仍有活力，但在夏季，经过2周时间后就无法证明有活虫卵了。
