Sakanashi M, Matsuzaki T, Nakasone J, Koyama T, Sakanashi M, Kukita I, Sakanashi M
Anaesth Intensive Care. 2002 Oct;30(5):570-7. doi: 10.1177/0310057X0203000504.
The venom obtained from Okinawan box-jellyfish (Habu-kurage), Chiropsalmus quadrigatus, produced increases in contractions of isolated rat right atrial preparations in a concentration-dependent manner without changes in a spontaneous beating rate. These increases in contractions were significantly inhibited by diltiazem and did not show tachyphylaxis. The venom also produced increases in contractions of isolated rat aortic ring preparations (endothelium-intact) in a concentration-dependent fashion, which were reproducible with repeated application and were significantly inhibited by diltiazem or heating. These increases in vascular contractions were weakened in endothelium-denuded preparations, and almost abolished in a calcium-free medium. On the other hand, the venom at higher concentrations diminished contractions of both myocardial and vascular preparations and did not show reproducibility. These results suggest that the Habu-kurage venom is heat-labile and may increase contractions of cardiac muscle and aortic smooth muscle by increasing calcium influx into muscle cells, and that the venom at higher concentrations may produce dysfunction of muscle contractile systems due to calcium overload.
从冲绳箱形水母（Habu-kurage，四手僧帽水母）中提取的毒液，能使离体大鼠右心房标本的收缩增强，且呈浓度依赖性，而自发搏动频率无变化。地尔硫䓬可显著抑制这些收缩增强，且未出现快速耐受性。该毒液还能使离体大鼠主动脉环标本（内皮完整）的收缩呈浓度依赖性增加，重复给药时可再现，且被地尔硫䓬或加热显著抑制。这些血管收缩增强在内皮剥脱的标本中减弱，在无钙培养基中几乎消失。另一方面，高浓度毒液会使心肌和血管标本的收缩减弱，且不可再现。这些结果表明，Habu-kurage毒液对热不稳定，可能通过增加钙流入肌肉细胞来增强心肌和主动脉平滑肌的收缩，且高浓度毒液可能因钙超载导致肌肉收缩系统功能障碍。
