Wang Ying, Yang Shu-xia, Tu Ping, Zhang Bo, Ma Sheng-qing
Department of Dermatology, The First Hospital of Peking University, Beijing 100034, People's Republic of China.
J Dermatol Sci. 2002 Nov;30(2):136-41. doi: 10.1016/s0923-1811(02)00074-9.
There is increasing evidence that parathyroid hormone (PTH) and PTH-related peptide (PTHrP) are involved in normal skin cell growth, influence the proliferation and differentiation of the epidermis and hair follicle. PTHrP and PTH/PTHrP receptor show prominent cutaneous expression, may exert important paracrine and/or autocrine functions. The expression of PTH/PTHrP receptor in different stages of hair cycle is unknown. Therefore, we examined the amount of PTH/PTHrP mRNA in C57BL/6 mice skin at different stages of hair cycle by relatively quantitative reverse transcription-polymerase chain reaction (RT-PCR), and investigated the localization of this receptor in mice skin by in situ hybridization. The expression of PTH/PTHrP receptor mRNA were higher in anagen, but significantly lower in catagen and telogen. Then, the PTH/PTHrP receptor mRNA was located in the inner root sheath (IRS) in anagen and catagen, but was not detected in telogen hair follicles, although it was expressed weakly in dermis. The variety of the PTH/PTHrP receptor mRNA expression during hair cycling suggest that PTH, PTHrP and their receptors might participate in the regulation of hair cycle in mice skin.
越来越多的证据表明，甲状旁腺激素（PTH）和甲状旁腺激素相关肽（PTHrP）参与正常皮肤细胞生长，影响表皮和毛囊的增殖与分化。PTHrP和PTH/PTHrP受体在皮肤中显著表达，可能发挥重要的旁分泌和/或自分泌功能。PTH/PTHrP受体在毛发生长周期不同阶段的表达情况尚不清楚。因此，我们通过相对定量逆转录-聚合酶链反应（RT-PCR）检测了C57BL/6小鼠皮肤在毛发生长周期不同阶段的PTH/PTHrP mRNA含量，并通过原位杂交研究了该受体在小鼠皮肤中的定位。PTH/PTHrP受体mRNA的表达在生长期较高，但在退行期和休止期显著降低。然后，PTH/PTHrP受体mRNA在生长期和退行期位于内根鞘（IRS），但在休止期毛囊中未检测到，尽管其在真皮中有微弱表达。毛发生长周期中PTH/PTHrP受体mRNA表达的变化表明，PTH、PTHrP及其受体可能参与小鼠皮肤毛发生长周期的调节。
