Seifer David B, DeJesus Vimaris, Hubbard Karen
Department of Obstetrics, Gynecology and Reproductive Sciences, Division of Reproductive Endocrinology and Infertility, UMDNJ-Robert Wood Johnson Medical School, New Brunswick, New Jersey 08901, USA.
Fertil Steril. 2002 Nov;78(5):1046-8. doi: 10.1016/s0015-0282(02)04214-0.
To test the hypothesis that mitochondrial DNA (mtDNA) deletions are more prevalent in granulosa cells from women of advanced reproductive age than from younger women undergoing IVF.
Granulosa cells screened for presence or absence of the 4977-bp deletion in human mtDNA.
University-based fertility clinic.
PATIENT(S): Twenty-four women divided equally between two groups: </=34 years old and >/=38 years old.
INTERVENTION(S): Patients were given gonadotropin stimulation in preparation for IVF with granulosa cells isolated at the time of follicular aspiration.
MAIN OUTCOME MEASURE(S): Presence or absence of the 4977-bp deletion in human mtDNA.
RESULT(S): Seven out of 12 women analyzed who were <38 years old and 0 out of 12 women who were >38 years old had normal mtDNA as indicated by the presence of the 4977-bp fragment.
CONCLUSION(S): These data suggest that women over the age of 38 have granulosa cells that contain a substantial decrease in the level of normal mitochondria as compared with women </=34 years.
检验如下假设，即与接受体外受精（IVF）的年轻女性相比，线粒体DNA（mtDNA）缺失在高龄生育女性的颗粒细胞中更为普遍。
筛选人线粒体DNA中4977碱基对缺失存在与否的颗粒细胞。
大学附属生育诊所。
24名女性平均分为两组：年龄≤34岁和年龄≥38岁。
患者接受促性腺激素刺激，为体外受精做准备，在卵泡抽吸时分离颗粒细胞。
人线粒体DNA中4977碱基对缺失的存在与否。
分析的12名年龄<38岁的女性中有7名以及12名年龄>38岁的女性中有0名的线粒体DNA正常，这由4977碱基对片段的存在表明。
这些数据表明，与年龄≤34岁的女性相比，38岁以上女性的颗粒细胞中正常线粒体水平大幅下降。
