Lütjen-Drecoll E, Sames K, Straub W, Krug K P
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1975;194(3):175-91. doi: 10.1007/BF00496874.
A case is briefly described in which a typical conjunctivitis lignosa appeared after the eye had suffered lime burns. In order to help clarify the morphological connection between mucopolysaccharide production and fiber development in the tumor tissue which occurred after the burn, samples were examined histologically, histochemically and with the use of the electron microscope. The tumor had a cartilage like consistency. Its structure could be devided into three regions. Region A is the pseudo-membrane. It has root like extensions which anchor it to the underlying tissue, and which morphologically appear partially homogeneous and partially fibrous. Blood cells and cell remnants are included in the tissue of the pseudomembrane. The histochemical examination of the pseudomembrane did not present a uniform picture. Along with small amounts of dermatan-sulfate and chondroitin-sulfate B the membrane probably contained a rather large amount of hyaluronic acid. The pseudomembrane borders on a granular tissue (Region B) which is distinguished by the wide metachromatic sheathes of the blood vessels found in it and the particularly large number of active fibroblasts along its edges. The silver impregnation method and the electron-microscopic examination showed that the vascular sheathes consist of bundles of reticular fibers which constitute a three-dimensional network. A similar sort of sheath was observed around the fibroblasts. Chondroitin-sulfate makes up the largest fraction of the mucopoly-saccharides near the fibers and appears particularly concentrated at the intersections of the fibers, although it is also diffusely distributed as well. Dermatan-sulfate (or heparan-sulfate) is found only in the mucopolysaccharide sheath of the fibers themselves. The deep region of the tumor (Region C) consists almost exclusively of blood vessels, their sprouts and the fibroblasts which, with their wide fibrous sheathes, almost fill the spaces between the blood vessels. The reticular fibers and their mucopolysaccharide sheathes have the same structure as that observed in Region B, The fact that the mucopolysaccharides did not appear in plaques but rather as bound primarily to the fibers is grounds for suggesting that a fiber development disorder, probably stemming from the pericytes and fibroblasts rich in ergastoplasm and fibrilles, could play the principle role in conjunctivitis lignosa. The cartilagen like consistency of the tumor could be a result of the arrangement of the fibers and their mucopolysaccharide sheathes. Brief remarks are included concerning the therapeutic consequences of the study.
简要描述了一个病例，在眼部遭受石灰烧伤后出现了典型的木质化结膜炎。为了有助于阐明烧伤后肿瘤组织中粘多糖产生与纤维发育之间的形态学联系，对样本进行了组织学、组织化学检查以及电子显微镜观察。肿瘤质地如软骨。其结构可分为三个区域。区域A为假膜。它有根状延伸部分，将其固定于下方组织，在形态上部分呈均匀状，部分呈纤维状。假膜组织中包含血细胞和细胞残余物。假膜的组织化学检查结果并不一致。除了少量的硫酸皮肤素和硫酸软骨素B外，该膜可能还含有相当大量的透明质酸。假膜与颗粒状组织（区域B）相邻，该颗粒状组织的特征在于其中发现的血管有宽阔的异染性鞘，且其边缘有特别大量的活跃成纤维细胞。银浸染法和电子显微镜检查表明，血管鞘由构成三维网络的网状纤维束组成。在成纤维细胞周围也观察到类似的鞘。硫酸软骨素在纤维附近的粘多糖中占最大比例，尤其集中在纤维交叉处，不过也呈弥散分布。硫酸皮肤素（或硫酸乙酰肝素）仅存在于纤维自身的粘多糖鞘中。肿瘤的深部区域（区域C）几乎完全由血管、其分支以及成纤维细胞组成，这些成纤维细胞及其宽阔的纤维鞘几乎填满了血管之间的空间。网状纤维及其粘多糖鞘与在区域B中观察到的结构相同。粘多糖并非以斑块形式出现，而是主要与纤维结合，这一事实表明，可能源于富含内质网和原纤维的周细胞和成纤维细胞的纤维发育障碍，可能在木质化结膜炎中起主要作用。肿瘤的软骨样质地可能是纤维及其粘多糖鞘排列的结果。文中还简要提及了该研究的治疗意义。
