长期摄入乙醇会使大鼠肠刷状缘膜中的磷脂酰乙醇胺N-甲基转移酶活性增加。
Phosphatidylethanolamine N-methyltransferase activity is increased in rat intestinal brush-border membrane by chronic ethanol ingestion.
作者信息
Furtado Valéria Cristina Soares, Takiya Christina Maeda, Braulio Valeria Bender
机构信息
Division of Nutrition and Metabolism, University Hospital Clementino Fraga Filho, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
出版信息
Alcohol Alcohol. 2002 Nov-Dec;37(6):561-5. doi: 10.1093/alcalc/37.6.561.
AIMS
Phosphatidylethanolamine N-methyltransferase (PEMT) catalyses the synthesis of phosphatidylcholine from phosphatidylethanolamine. The aim of this study was to evaluate the effect of chronic ethanol ingestion on PEMT activity in the jejunal brush-border membrane (BBM) of adequately nourished rats.
METHODS
For this purpose, rats were fed a liquid diet containing ethanol [ethanol-fed group (EFG)] or an isocaloric liquid diet without ethanol [pair-fed group (PFG)] for 4 weeks. Diet ingestion, body weight, nitrogen balance and urinary creatinine excretion were monitored during the experimental period, and serum transferrin levels were determined at the end. BBM was isolated for the determination of PEMT activity.
RESULTS
PEMT activity was significantly increased in the jejunal BBM of the EFG. Nutritional parameters, however, did not differ between groups.
CONCLUSIONS
The increase in PEMT activity may be attributed exclusively to chronic ethanol ingestion, since a major nutritional deficit was excluded.
目的
磷脂酰乙醇胺N-甲基转移酶（PEMT）催化由磷脂酰乙醇胺合成磷脂酰胆碱。本研究的目的是评估长期摄入乙醇对营养充足的大鼠空肠刷状缘膜（BBM）中PEMT活性的影响。
方法
为此，将大鼠分为两组，一组喂食含乙醇的液体饲料[乙醇喂养组（EFG）]，另一组喂食等热量不含乙醇的液体饲料[配对喂养组（PFG）]，持续4周。在实验期间监测饮食摄入量、体重、氮平衡和尿肌酐排泄，并在实验结束时测定血清转铁蛋白水平。分离BBM以测定PEMT活性。
结果
EFG组空肠BBM中的PEMT活性显著增加。然而，两组之间的营养参数没有差异。
结论
由于排除了主要的营养缺乏因素，PEMT活性的增加可能完全归因于长期摄入乙醇。
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