al-Balool F, Debnam E S
Department of Physiology, Royal Free Hospital School of Medicine, London.
Q J Exp Physiol. 1989 Sep;74(5):751-3. doi: 10.1113/expphysiol.1989.sp003327.
Rats were pair-fed for 28-32 days a nutritionally adequate liquid control diet or an isocaloric diet containing ethanol (5% v/v). Brush-border membrane vesicles were prepared from jejunal mucosa and Na+-dependent D-glucose accumulation was measured using sugar concentrations of 0.2-1.0 mmol l-1. In separate experiments the effects of acute ethanol exposure on glucose uptake by vesicles prepared from chow-fed rats were determined. Chronic ethanol ingestion enhanced Na+-gradient-dependent glucose uptake. In contrast, incubation of vesicles with alcohol (4% v/v), reduced active uptake of the sugar.
将大鼠以营养充足的液体对照饮食或含乙醇（5%体积/体积）的等热量饮食配对喂养28 - 32天。从空肠黏膜制备刷状缘膜囊泡，并使用0.2 - 1.0 mmol l-1的糖浓度测量Na+依赖的D - 葡萄糖积累。在单独的实验中，测定了急性乙醇暴露对从正常饮食喂养的大鼠制备的囊泡摄取葡萄糖的影响。长期摄入乙醇增强了Na+梯度依赖的葡萄糖摄取。相反，将囊泡与酒精（4%体积/体积）一起孵育会降低糖的主动摄取。
