Arraztoa Jose A, Monget Philippe, Bondy Carolyn, Zhou Jian
Developmental Endocrinology Branch, National Institute of Child Health and Human Development/NIH, Building 10, Room 10N262, Bethesda, MD 20892, USA.
J Clin Endocrinol Metab. 2002 Nov;87(11):5220-8. doi: 10.1210/jc.2002-020407.
IGFs and IGF-binding proteins (IGFBPs) are thought to play important roles in ovarian follicular growth and selection. To elucidate the role of IGFBPs in primate ovarian function, we analyzed IGFBP mRNA expression patterns in ovaries from mid-cycle rhesus monkeys using in situ hybridization. IGFBP-1 mRNA was concentrated in theca-interstitial cells and was present at low levels in granulosa cells of atretic follicles. IGFBP-2 mRNA was expressed in the ovarian surface epithelium and granulosa cells of all antral follicles, including obviously atretic as well as dominant follicles. IGFBP-3 mRNA was localized in oocytes and in the ovarian vascular endothelium; this mRNA was also concentrated in the superficial cortical stroma in which it was distinctly more abundant in the nondominant ovary. Granulosa cells of mature dominant and ovulatory follicles selectively expressed IGFBP-5 mRNA. IGFBP-5 mRNA was also widely expressed in the ovarian stroma, in which, in contrast to IGFBP-3, it was distinctly more abundant in dominant, compared with nondominant, ovary. IGFBP-6 mRNA was present at low levels in the ovary interstitium and theca externa and was more abundant in the ovary surface epithelium. These novel data reveal distinctive cellular expression patterns for IGFBPs 1, 2, 3, 5, and 6 in the nonhuman primate ovary, suggesting distinct roles for each binding protein in ovarian function.
胰岛素样生长因子（IGFs）和胰岛素样生长因子结合蛋白（IGFBPs）被认为在卵巢卵泡生长和选择中发挥重要作用。为了阐明IGFBPs在灵长类动物卵巢功能中的作用，我们使用原位杂交技术分析了恒河猴月经周期中期卵巢中IGFBP mRNA的表达模式。IGFBP - 1 mRNA集中在卵泡膜间质细胞中，在闭锁卵泡的颗粒细胞中含量较低。IGFBP - 2 mRNA在卵巢表面上皮和所有窦状卵泡的颗粒细胞中表达，包括明显闭锁的卵泡和优势卵泡。IGFBP - 3 mRNA定位于卵母细胞和卵巢血管内皮细胞中；这种mRNA也集中在浅表皮质基质中，在非优势卵巢中明显更为丰富。成熟优势卵泡和排卵卵泡的颗粒细胞选择性表达IGFBP - 5 mRNA。IGFBP - 5 mRNA也广泛表达于卵巢基质中，与IGFBP - 3相反，与非优势卵巢相比，在优势卵巢中明显更为丰富。IGFBP - 6 mRNA在卵巢间质和卵泡膜外层中含量较低，在卵巢表面上皮中更为丰富。这些新数据揭示了IGFBPs 1、2、3、5和6在非人类灵长类动物卵巢中独特的细胞表达模式，表明每种结合蛋白在卵巢功能中具有不同的作用。
