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1
Peptides containing cyclin/Cdk-nuclear localization signal motifs derived from viral initiator proteins bind to DNA when unphosphorylated.含有源自病毒起始蛋白的细胞周期蛋白/细胞周期蛋白依赖性激酶核定位信号基序的肽在未磷酸化时与DNA结合。
J Virol. 2002 Dec;76(23):11785-92. doi: 10.1128/jvi.76.23.11785-11792.2002.
2
Role of flanking sequences and phosphorylation in the recognition of the simian-virus-40 large T-antigen nuclear localization sequences by importin-alpha.侧翼序列和磷酸化在输入蛋白α识别猿猴病毒40大T抗原核定位序列中的作用
Biochem J. 2003 Oct 15;375(Pt 2):339-49. doi: 10.1042/BJ20030510.
3
Mitogen-activated protein kinases activate the nuclear localization sequence of human papillomavirus type 11 E1 DNA helicase to promote efficient nuclear import.丝裂原活化蛋白激酶激活人乳头瘤病毒11型E1 DNA解旋酶的核定位序列，以促进有效的核输入。
J Virol. 2007 May;81(10):5066-78. doi: 10.1128/JVI.02480-06. Epub 2007 Mar 7.
4
Phosphorylation of simian virus 40 T antigen on Thr 124 selectively promotes double-hexamer formation on subfragments of the viral core origin.猿猴病毒40 T抗原在苏氨酸124位点的磷酸化选择性地促进病毒核心起始子亚片段上双六聚体的形成。
J Virol. 2000 Sep;74(18):8601-13. doi: 10.1128/jvi.74.18.8601-8613.2000.
5
Cyclin/CDK regulates the nucleocytoplasmic localization of the human papillomavirus E1 DNA helicase.细胞周期蛋白/细胞周期蛋白依赖性激酶调节人乳头瘤病毒E1 DNA解旋酶的核质定位。
J Virol. 2004 Dec;78(24):13954-65. doi: 10.1128/JVI.78.24.13954-13965.2004.
6
Interaction of nucleolar protein B23 with peptides related to nuclear localization signals.核仁蛋白B23与核定位信号相关肽段的相互作用。
Biochemistry. 1995 Jun 27;34(25):8037-42. doi: 10.1021/bi00025a009.
7
Bovine papillomavirus E1 protein can, by itself, efficiently drive multiple rounds of DNA synthesis in vitro.牛乳头瘤病毒E1蛋白自身就能在体外有效地驱动多轮DNA合成。
J Virol. 1995 May;69(5):3201-5. doi: 10.1128/JVI.69.5.3201-3205.1995.
8
Phosphorylation of bovine papillomavirus E1 by the protein kinase CK2 near the nuclear localization signal does not influence subcellular distribution of the protein in dividing cells.蛋白激酶CK2在牛乳头瘤病毒E1的核定位信号附近对其进行磷酸化，这并不影响该蛋白在分裂细胞中的亚细胞分布。
Arch Virol. 2016 Jan;161(1):165-9. doi: 10.1007/s00705-015-2641-6. Epub 2015 Oct 14.
9
Quantitative analysis of the binding of simian virus 40 large T antigen to DNA.猿猴病毒40大T抗原与DNA结合的定量分析。
J Virol. 2007 Sep;81(17):9162-74. doi: 10.1128/JVI.00384-07. Epub 2007 Jun 27.
10
The BRCA-1 binding protein BRAP2 is a novel, negative regulator of nuclear import of viral proteins, dependent on phosphorylation flanking the nuclear localization signal.BRCA-1 结合蛋白 BRAP2 是一种新型的病毒蛋白核输入的负调节剂，依赖于核定位信号侧翼的磷酸化。
FASEB J. 2010 May;24(5):1454-66. doi: 10.1096/fj.09-136564. Epub 2009 Dec 29.

引用本文的文献

1
Quantitative analysis of the binding of simian virus 40 large T antigen to DNA.猿猴病毒40大T抗原与DNA结合的定量分析。
J Virol. 2007 Sep;81(17):9162-74. doi: 10.1128/JVI.00384-07. Epub 2007 Jun 27.
2
Stability and function of JC virus large T antigen and T' proteins are altered by mutation of their phosphorylated threonine 125 residues.JC病毒大T抗原和T'蛋白的稳定性及功能因125位苏氨酸磷酸化残基的突变而改变。
J Virol. 2006 Mar;80(5):2083-91. doi: 10.1128/JVI.80.5.2083-2091.2006.
3
Interactions required for binding of simian virus 40 T antigen to the viral origin and molecular modeling of initial assembly events.猿猴病毒40 T抗原与病毒起源结合所需的相互作用及初始组装事件的分子建模。
J Virol. 2004 Mar;78(6):2921-34. doi: 10.1128/jvi.78.6.2921-2934.2004.

本文引用的文献

1
SV40 large T antigen hexamer structure: domain organization and DNA-induced conformational changes.SV40大T抗原六聚体结构：结构域组织与DNA诱导的构象变化
Curr Biol. 2002 Mar 19;12(6):472-6. doi: 10.1016/s0960-9822(02)00696-6.
2
Concerted evolution of structure and function in a miniature protein.一种微型蛋白质中结构与功能的协同进化。
J Am Chem Soc. 2001 Mar 28;123(12):2929-30. doi: 10.1021/ja0056668.
3
Finding nuclear localization signals.寻找核定位信号。
EMBO Rep. 2000 Nov;1(5):411-5. doi: 10.1093/embo-reports/kvd092.
4
Methods for studying interactions between Simian virus 40 T-antigen and the viral origin of replication.
Methods Mol Biol. 2001;165:49-67. doi: 10.1385/1-59259-117-5:49.
5
Phosphorylation-dependent prolyl isomerization: a novel signaling regulatory mechanism.磷酸化依赖性脯氨酰异构化：一种新型信号调节机制。
Cell Mol Life Sci. 1999 Nov 30;56(9-10):788-806. doi: 10.1007/s000180050026.
6
SV40 large T antigen functions in DNA replication and transformation.SV40大T抗原在DNA复制和转化过程中发挥作用。
Adv Virus Res. 2000;55:75-134. doi: 10.1016/s0065-3527(00)55002-7.
7
Regulation of chromosome replication.染色体复制的调控
Annu Rev Biochem. 2000;69:829-80. doi: 10.1146/annurev.biochem.69.1.829.
8
Phosphorylation of simian virus 40 T antigen on Thr 124 selectively promotes double-hexamer formation on subfragments of the viral core origin.猿猴病毒40 T抗原在苏氨酸124位点的磷酸化选择性地促进病毒核心起始子亚片段上双六聚体的形成。
J Virol. 2000 Sep;74(18):8601-13. doi: 10.1128/jvi.74.18.8601-8613.2000.
9
The simian virus 40 core origin contains two separate sequence modules that support T-antigen double-hexamer assembly.猿猴病毒40核心起源包含两个独立的序列模块，它们支持T抗原双六聚体组装。
J Virol. 2000 Sep;74(18):8589-600. doi: 10.1128/jvi.74.18.8589-8600.2000.
10
Development of a functional backbone cyclic mimetic of the HIV-1 Tat arginine-rich motif.HIV-1反式激活因子富含精氨酸基序的功能性骨架环模拟物的开发。
J Biol Chem. 2000 Aug 4;275(31):23783-9. doi: 10.1074/jbc.M002200200.

含有源自病毒起始蛋白的细胞周期蛋白/细胞周期蛋白依赖性激酶核定位信号基序的肽在未磷酸化时与DNA结合。

Peptides containing cyclin/Cdk-nuclear localization signal motifs derived from viral initiator proteins bind to DNA when unphosphorylated.

作者信息

Kim Ronald J, Moine Stephanie, Reese Danielle K, Bullock Peter A

机构信息

Department of Biochemistry, Tufts University School of Medicine, Boston, Massachusetts 02111, USA.

出版信息

J Virol. 2002 Dec;76(23):11785-92. doi: 10.1128/jvi.76.23.11785-11792.2002.

DOI:10.1128/jvi.76.23.11785-11792.2002
PMID:12414920
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC136914/
Abstract

A single phosphorylation event at T-antigen residue Thr124 regulates initiation of simian virus 40 DNA replication. To explore this regulatory process, a series of peptides were synthesized, centered on Thr124. These peptides contain a nuclear localization signal (NLS) and a recognition site for cyclin/Cdk kinases. When unphosphorylated, the "CDK/NLS" peptides inhibit T-antigen assembly and bind non-sequence specifically to DNA. However, these activities are greatly reduced upon phosphorylation of Thr124. Similar results were obtained by using peptides derived from the CDK/NLS region of bovine papillomavirus E1. Related studies indicate that residues in the NLS bind to DNA, whereas those in the CDK motif regulate binding. These findings are discussed in terms of the control of T-antigen double hexamer assembly and initiation of viral replication.

摘要

T抗原残基苏氨酸124处的单个磷酸化事件调节猿猴病毒40 DNA复制的起始。为了探究这一调节过程，合成了一系列以苏氨酸124为中心的肽段。这些肽段包含一个核定位信号（NLS）和一个细胞周期蛋白/细胞周期蛋白依赖性激酶（Cdk）的识别位点。未磷酸化时，“CDK/NLS”肽段抑制T抗原组装并与DNA进行非序列特异性结合。然而，苏氨酸124磷酸化后，这些活性会大大降低。使用源自牛乳头瘤病毒E1的CDK/NLS区域的肽段也获得了类似结果。相关研究表明，NLS中的残基与DNA结合，而CDK基序中的残基调节结合。将根据T抗原双六聚体组装的控制和病毒复制的起始来讨论这些发现。