来自原发性渗出性淋巴瘤的卡波西肉瘤相关疱疹病毒K12转录本含有复杂的重复元件，经过剪接，并从一个新的启动子起始转录。
The Kaposi's sarcoma-associated herpesvirus K12 transcript from a primary effusion lymphoma contains complex repeat elements, is spliced, and initiates from a novel promoter.
作者信息
Li Hong, Komatsu Takashi, Dezube Bruce J, Kaye Kenneth M
机构信息
Department of Medicine, Channing Laboratory, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02215, USA.
出版信息
J Virol. 2002 Dec;76(23):11880-8. doi: 10.1128/jvi.76.23.11880-11888.2002.
Abstract
Kaposi's sarcoma-associated herpesvirus (KSHV) latently infects KS tumors, primary effusion lymphomas (PELs), and PEL cell lines. K12 (T0.7) is the most abundant transcript expressed in latent KSHV infection. We characterize here the K12 transcript from a PEL tumor prior to passage in cell culture. The PEL tumor KSHV K12 transcript contained additional complex nucleotide repeat elements compared to the previously described K12 message of the BCBL-1 PEL cell line. The PEL tumor lacked kaposin B, the predominant BCBL-1 K12 protein product, but encoded kaposins A and C. The K12 transcript was spliced and the splicing event occurred in all KSHV isolates tested. The 5' end of the K12 transcript was mapped by 5' RACE (rapid amplification of cDNA ends) and S1 nuclease protection assays and was at the site of an active promoter. This work demonstrates that the K12 transcript contains variable, complex repeat elements, is spliced and is expressed from a novel KSHV promoter.
摘要
卡波西肉瘤相关疱疹病毒（KSHV）潜伏感染卡波西肉瘤肿瘤、原发性渗出性淋巴瘤（PEL）以及PEL细胞系。K12（T0.7）是潜伏性KSHV感染中表达量最高的转录本。我们在此对一株未经细胞培养传代的PEL肿瘤中的K12转录本进行了特征分析。与先前描述的BCBL - 1 PEL细胞系的K12信息相比，该PEL肿瘤的KSHV K12转录本包含额外的复杂核苷酸重复元件。该PEL肿瘤缺乏卡波辛B（BCBL - 1 K12的主要蛋白产物），但编码卡波辛A和C。K12转录本发生了剪接，且剪接事件在所有测试的KSHV分离株中均有发生。通过5' RACE（cDNA末端快速扩增）和S1核酸酶保护试验对K12转录本的5'端进行了定位，其位于一个活性启动子位点。这项工作表明，K12转录本包含可变的复杂重复元件，发生了剪接，并由一个新的KSHV启动子表达。
相似文献
1
The Kaposi's sarcoma-associated herpesvirus K12 transcript from a primary effusion lymphoma contains complex repeat elements, is spliced, and initiates from a novel promoter.来自原发性渗出性淋巴瘤的卡波西肉瘤相关疱疹病毒K12转录本含有复杂的重复元件，经过剪接，并从一个新的启动子起始转录。
J Virol. 2002 Dec;76(23):11880-8. doi: 10.1128/jvi.76.23.11880-11888.2002.
2
Characterization of the human herpesvirus 8 (Kaposi's sarcoma-associated herpesvirus) oncogene, kaposin (ORF K12).人类疱疹病毒8型（卡波西肉瘤相关疱疹病毒）癌基因卡波辛（开放阅读框K12）的特征分析
J Clin Virol. 2000 May;16(3):203-13. doi: 10.1016/s1386-6532(99)00081-5.
3
A complex translational program generates multiple novel proteins from the latently expressed kaposin (K12) locus of Kaposi's sarcoma-associated herpesvirus.一个复杂的翻译程序从卡波西肉瘤相关疱疹病毒潜在表达的卡波西蛋白（K12）基因座产生多种新型蛋白质。
J Virol. 1999 Jul;73(7):5722-30. doi: 10.1128/JVI.73.7.5722-5730.1999.
4
Identification of a spliced gene from Kaposi's sarcoma-associated herpesvirus encoding a protein with similarities to latent membrane proteins 1 and 2A of Epstein-Barr virus.从卡波西肉瘤相关疱疹病毒中鉴定出一个剪接基因，该基因编码一种与爱泼斯坦-巴尔病毒的潜伏膜蛋白1和2A相似的蛋白质。
J Virol. 1999 Aug;73(8):6953-63. doi: 10.1128/JVI.73.8.6953-6963.1999.
5
Transcription pattern of human herpesvirus 8 open reading frame K3 in primary effusion lymphoma and Kaposi's sarcoma.人疱疹病毒8型开放阅读框K3在原发性渗出性淋巴瘤和卡波西肉瘤中的转录模式
J Virol. 2001 Aug;75(15):7161-74. doi: 10.1128/JVI.75.15.7161-7174.2001.
6
Identification of kaposin (open reading frame K12) as a human herpesvirus 8 (Kaposi's sarcoma-associated herpesvirus) transforming gene.鉴定卡波西蛋白（开放阅读框K12）为人类疱疹病毒8型（卡波西肉瘤相关疱疹病毒）的转化基因。
J Virol. 1998 Jun;72(6):4980-8. doi: 10.1128/JVI.72.6.4980-4988.1998.
7
Expression of Kaposi's sarcoma-associated herpesvirus G protein-coupled receptor monocistronic and bicistronic transcripts in primary effusion lymphomas.卡波西肉瘤相关疱疹病毒G蛋白偶联受体单顺反子和双顺反子转录本在原发性渗出性淋巴瘤中的表达
Virology. 2001 Aug 15;287(1):62-70. doi: 10.1006/viro.2001.1016.
8
Kaposi's sarcoma-associated herpesvirus contains G protein-coupled receptor and cyclin D homologs which are expressed in Kaposi's sarcoma and malignant lymphoma.卡波西肉瘤相关疱疹病毒含有G蛋白偶联受体和细胞周期蛋白D同源物，它们在卡波西肉瘤和恶性淋巴瘤中表达。
J Virol. 1996 Nov;70(11):8218-23. doi: 10.1128/JVI.70.11.8218-8223.1996.
9
Differential viral protein expression in Kaposi's sarcoma-associated herpesvirus-infected diseases: Kaposi's sarcoma, primary effusion lymphoma, and multicentric Castleman's disease.卡波西肉瘤相关疱疹病毒感染疾病中的病毒蛋白差异表达：卡波西肉瘤、原发性渗出性淋巴瘤和多中心Castleman病。
Am J Pathol. 2000 Mar;156(3):743-9. doi: 10.1016/S0002-9440(10)64940-1.
10
Pathological Features of Kaposi's Sarcoma-Associated Herpesvirus Infection.卡波西肉瘤相关疱疹病毒感染的病理特征。
Adv Exp Med Biol. 2018;1045:357-376. doi: 10.1007/978-981-10-7230-7_16.
引用本文的文献
1
Unraveling the Kaposi Sarcoma-Associated Herpesvirus (KSHV) Lifecycle: An Overview of Latency, Lytic Replication, and KSHV-Associated Diseases.解析卡波西肉瘤相关疱疹病毒（KSHV）的生命周期：潜伏、裂解复制及KSHV相关疾病概述
Viruses. 2025 Jan 26;17(2):177. doi: 10.3390/v17020177.
2
Variation within major internal repeats of KSHV in vivo.卡波西肉瘤相关疱疹病毒（KSHV）体内主要内部重复序列的变异
Virus Evol. 2023 May 22;9(1):vead034. doi: 10.1093/ve/vead034. eCollection 2023.
3
Evidence for Multiple Subpopulations of Herpesvirus-Latently Infected Cells.证据表明潜伏感染细胞存在多种疱疹病毒亚群。
mBio. 2022 Feb 22;13(1):e0347321. doi: 10.1128/mbio.03473-21. Epub 2022 Jan 4.
4
Quantitative RNAseq analysis of Ugandan KS tumors reveals KSHV gene expression dominated by transcription from the LTd downstream latency promoter.对乌干达 KS 肿瘤的定量 RNAseq 分析显示，KSHV 基因表达主要由 LTd 下游潜伏期启动子的转录驱动。
PLoS Pathog. 2018 Dec 17;14(12):e1007441. doi: 10.1371/journal.ppat.1007441. eCollection 2018 Dec.
5
Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Associated Disease in the AIDS Patient: An Update.艾滋病患者中卡波西肉瘤相关疱疹病毒（KSHV）相关疾病的最新进展
Cancer Treat Res. 2019;177:63-80. doi: 10.1007/978-3-030-03502-0_3.
6
Quantitative Analysis of the KSHV Transcriptome Following Primary Infection of Blood and Lymphatic Endothelial Cells.血液和淋巴管内皮细胞初次感染后卡波西肉瘤相关疱疹病毒转录组的定量分析
Pathogens. 2017 Mar 19;6(1):11. doi: 10.3390/pathogens6010011.
7
Expression of the Antisense-to-Latency Transcript Long Noncoding RNA in Kaposi's Sarcoma-Associated Herpesvirus.卡波西肉瘤相关疱疹病毒中潜伏期转录反义长链非编码RNA的表达
J Virol. 2017 Jan 31;91(4). doi: 10.1128/JVI.01698-16. Print 2017 Feb 15.
8
Identification of Novel Kaposi's Sarcoma-Associated Herpesvirus Orf50 Transcripts: Discovery of New RTA Isoforms with Variable Transactivation Potential.新型卡波西肉瘤相关疱疹病毒Orf50转录本的鉴定：具有可变反式激活潜能的新型RTA亚型的发现
J Virol. 2016 Dec 16;91(1). doi: 10.1128/JVI.01434-16. Print 2017 Jan 1.
9
Nuclear Innate Immune DNA Sensor IFI16 Is Degraded during Lytic Reactivation of Kaposi's Sarcoma-Associated Herpesvirus (KSHV): Role of IFI16 in Maintenance of KSHV Latency.核内先天性免疫DNA传感器IFI16在卡波西肉瘤相关疱疹病毒（KSHV）裂解激活过程中被降解：IFI16在维持KSHV潜伏中的作用。
J Virol. 2016 Sep 12;90(19):8822-41. doi: 10.1128/JVI.01003-16. Print 2016 Oct 1.
10
Chromatinization of the KSHV Genome During the KSHV Life Cycle.在 KSHV 生命周期中 KSHV 基因组的染色质化。
Cancers (Basel). 2015 Jan 14;7(1):112-42. doi: 10.3390/cancers7010112.
本文引用的文献
1
Human herpesvirus-8 encoded Kaposin: subcellular localization using immunofluorescence and biochemical approaches.人类疱疹病毒8型编码的卡波西蛋白：采用免疫荧光和生化方法进行亚细胞定位
DNA Cell Biol. 2002 Mar;21(3):151-62. doi: 10.1089/10445490252925413.
2
K15 protein of Kaposi's sarcoma-associated herpesvirus is latently expressed and binds to HAX-1, a protein with antiapoptotic function.卡波西肉瘤相关疱疹病毒的K15蛋白呈潜伏性表达，并与具有抗凋亡功能的蛋白HAX-1结合。
J Virol. 2002 Jan;76(2):802-16. doi: 10.1128/jvi.76.2.802-816.2002.
3
Signaling by human herpesvirus 8 kaposin A through direct membrane recruitment of cytohesin-1.人疱疹病毒8型卡波西蛋白A通过直接膜募集细胞衔接蛋白-1进行信号传导。
Mol Cell. 2001 Apr;7(4):833-43. doi: 10.1016/s1097-2765(01)00227-1.
4
Latently expressed human herpesvirus 8-encoded interferon regulatory factor 2 inhibits double-stranded RNA-activated protein kinase.潜伏表达的人疱疹病毒8编码的干扰素调节因子2抑制双链RNA激活的蛋白激酶。
J Virol. 2001 Mar;75(5):2345-52. doi: 10.1128/JVI.75.5.2345-2352.2001.
5
Kaposi's sarcoma-associated herpesvirus LANA2 is a B-cell-specific latent viral protein that inhibits p53.卡波西肉瘤相关疱疹病毒LANA2是一种B细胞特异性潜伏病毒蛋白，可抑制p53。
J Virol. 2001 Jan;75(1):429-38. doi: 10.1128/JVI.75.1.429-438.2001.
6
Impaired CTL recognition of cells latently infected with Kaposi's sarcoma-associated herpes virus.
J Immunol. 2000 Aug 15;165(4):2077-83. doi: 10.4049/jimmunol.165.4.2077.
7
Characterization of the human herpesvirus 8 (Kaposi's sarcoma-associated herpesvirus) oncogene, kaposin (ORF K12).人类疱疹病毒8型（卡波西肉瘤相关疱疹病毒）癌基因卡波辛（开放阅读框K12）的特征分析
J Clin Virol. 2000 May;16(3):203-13. doi: 10.1016/s1386-6532(99)00081-5.
8
9
Identification of a spliced gene from Kaposi's sarcoma-associated herpesvirus encoding a protein with similarities to latent membrane proteins 1 and 2A of Epstein-Barr virus.从卡波西肉瘤相关疱疹病毒中鉴定出一个剪接基因，该基因编码一种与爱泼斯坦-巴尔病毒的潜伏膜蛋白1和2A相似的蛋白质。
J Virol. 1999 Aug;73(8):6953-63. doi: 10.1128/JVI.73.8.6953-6963.1999.
10
Comparison of genetic variability at multiple loci across the genomes of the major subtypes of Kaposi's sarcoma-associated herpesvirus reveals evidence for recombination and for two distinct types of open reading frame K15 alleles at the right-hand end.卡波西肉瘤相关疱疹病毒主要亚型基因组中多个位点的遗传变异性比较揭示了重组证据以及右手端存在两种不同类型的开放阅读框K15等位基因。
J Virol. 1999 Aug;73(8):6646-60. doi: 10.1128/JVI.73.8.6646-6660.1999.
Zotero 插件