Cesarman E, Nador R G, Bai F, Bohenzky R A, Russo J J, Moore P S, Chang Y, Knowles D M
Department of Pathology, The New York Hospital-Cornell Medical Center, New York 10021, USA.
J Virol. 1996 Nov;70(11):8218-23. doi: 10.1128/JVI.70.11.8218-8223.1996.
A new human herpesvirus was recently identified in all forms of Kaposi's sarcoma (Kaposi's sarcoma-associated herpesvirus [KSHV] or human herpesvirus 8), as well as in primary effusion (body cavity-based) lymphomas (PELs). A 12.3-kb-long KSHV clone was obtained from a PEL genomic library. Sequencing of this clone revealed extensive homology and colinearity with the right end of the herpesvirus saimiri (HVS) genome and more limited homology to the left end of the Epstein-Barr virus genome. Four open reading frames (ORFs) were sequenced and characterized; these are homologous to the following viral and/or cellular genes: (i) Epstein-Barr virus membrane antigen p140 and HVS p160, (ii) HVS and cellular type D cyclins, (iii) HVS and cellular G protein-coupled receptors, and (iv) HVS. Since there is considerable evidence that cyclin D1 and some G protein-coupled receptors contribute to the development of specific cancers, the presence of KSHV homologs of these genes provides support for a role for KSHV in malignant transformation. All ORFs identified are transcribed in PELs and Kaposi's sarcoma tissues, further suggesting an active role for KSHV in these diseases.
最近在所有类型的卡波西肉瘤（卡波西肉瘤相关疱疹病毒[KSHV]或人类疱疹病毒8）以及原发性渗出性（基于体腔）淋巴瘤（PEL）中发现了一种新的人类疱疹病毒。从一个PEL基因组文库中获得了一个长度为12.3 kb的KSHV克隆。对该克隆进行测序后发现，它与猴疱疹病毒（HVS）基因组的右端具有广泛的同源性和共线性，而与爱泼斯坦-巴尔病毒基因组的左端同源性较低。对四个开放阅读框（ORF）进行了测序和特征分析；它们与以下病毒和/或细胞基因同源：（i）爱泼斯坦-巴尔病毒膜抗原p140和HVS p160，（ii）HVS和细胞D型细胞周期蛋白，（iii）HVS和细胞G蛋白偶联受体，以及（iv）HVS。由于有大量证据表明细胞周期蛋白D1和一些G蛋白偶联受体与特定癌症的发生有关，这些基因的KSHV同源物的存在为KSHV在恶性转化中的作用提供了支持。所有鉴定出的ORF在PEL和卡波西肉瘤组织中均有转录，这进一步表明KSHV在这些疾病中发挥着积极作用。
