[对从巴塔哥尼亚土壤中分离出的菌株中的芳烃分解代谢基因的分析]

[Analysis of aromatic hydrocarbon catabolic genes in strains isolated from soil in Patagonia].

作者信息

Vacca G S, Kiesel B, Wünsche L, Pucci O H

机构信息

CEIMA, Facultad de Ciencias Naturales, Universidad Nacional de la Patagonia, Km 4, 9000 Comodoro Rivadavia, Chubut, Argentina.

出版信息

Rev Argent Microbiol. 2002 Jul-Sep;34(3):138-49.

PMID:12415896

Abstract

Seven strains belonging to genus Pseudomonas were isolated from an enrichment with hydrocarbon mixtures. Tests for enzyme activities showed that five strains used predominantly the catabolic meta-pathway for aromatic hydrocarbon degradation. Furthermore, the xylE gene which encodes a catechol 2,3-dioxygenase was amplified by PCR, and in two strains the nahAc gene, a key enzyme for naphthalene catabolism, was also found. The xylE gene might be a good marker to identify aromatic hydrocarbon degrading bacteria in soils from Patagonia.

摘要

从富含碳氢化合物混合物的富集培养物中分离出7株假单胞菌属菌株。酶活性测试表明，5株菌株主要利用分解代谢间位途径降解芳香烃。此外，通过PCR扩增了编码儿茶酚2,3-双加氧酶的xylE基因，并且在两株菌株中还发现了萘分解代谢的关键酶nahAc基因。xylE基因可能是鉴定巴塔哥尼亚土壤中芳香烃降解细菌的良好标记。