[土壤DNA中萘代谢关键基因的鉴定]

[Identification of the key genes of naphthalene catabolism in soil DNA].

作者信息

Mavrodi D V, Kovalenko N P, Sokolov S L, Parfeniuk V G, Kosheleva I A, Boronin A M

机构信息

Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow Oblast, 142290 Russia.

出版信息

Mikrobiologiia. 2003 Sep-Oct;72(5):672-80.

PMID:14679907

Abstract

The key genes nahAc and xylE of the naphthalene catabolism of fluorescent Pseudomonas spp. in the total soil DNA samples were detected by the polymerase chain reaction (PCR) technique. The collection of fluorescent Pseudomonas spp. was screened for the occurrence of these genes. The results obtained show the possibility of using this approach in the goal-directed search for plasmid-containing naphthalene-degrading fluorescent pseudomonads in soil. The distribution of the naphthalene catabolism genes in soils contaminated with creosote and petroleum products was also studied.

摘要

采用聚合酶链反应（PCR）技术检测了土壤总DNA样本中荧光假单胞菌属萘代谢的关键基因nahAc和xylE。对荧光假单胞菌属的菌株进行筛选，以检测这些基因的存在情况。所得结果表明，这种方法有可能用于有针对性地在土壤中寻找含质粒的萘降解荧光假单胞菌。还研究了杂酚油和石油产品污染土壤中萘代谢基因的分布情况。