Ritacco V, López B, Paul R, Reniero A, Di Lonardo M, Casimir L, Togneri A, Kaufman S, Barrera L
Servicio de Micobacterias, INEI ANLIS Carlos G. Malbrán, Av. Vélez Sarsfield 563, 1281 Buenos Aires, Argentina.
Rev Argent Microbiol. 2002 Jul-Sep;34(3):163-6.
Fifteen episodes of Mycobacterium tuberculosis laboratory cross-contamination suspected between 1996 and 2001 at 6 laboratories in Buenos Aires City and suburbs were investigated by IS6110 RFLP. Thirteen episodes were confirmed. Even though BACTEC 460 produced the highest number of confirmed episodes in a single laboratory, the most extended one occurred while employing conventional culture procedures in solid medium. The double repetitive element-polymerase chain reaction (DRE-PCR) was applied to 8 of these episodes and produced concordant results with those of the RFLP. The DRE-PCR appears to be a valuable tool for the prompt identification of false positive cultures. The timely rectification of defects in laboratory protocols can avert false diagnoses of tuberculosis and unnecessary prolonged treatments.
1996年至2001年间，对布宜诺斯艾利斯市及郊区6家实验室疑似发生的15起结核分枝杆菌实验室交叉污染事件进行了IS6110限制性片段长度多态性（RFLP）调查。其中13起事件得到确认。尽管BACTEC 460在单个实验室中产生的确认事件数量最多，但最广泛的交叉污染事件发生在使用固体培养基的传统培养程序时。对其中8起事件应用了双重复元件聚合酶链反应（DRE-PCR），其结果与RFLP结果一致。DRE-PCR似乎是快速鉴定假阳性培养物的有价值工具。及时纠正实验室操作规程中的缺陷可避免结核病的误诊和不必要的长期治疗。
