Van Duin J M, Pijnenburg J E, van Rijswoud C M, de Haas P E, Hendriks W D, van Soolingen D
Regional Medical Microbiological Laboratory, Zuiderziekenhuis, Rotterdam, The Netherlands.
Int J Tuberc Lung Dis. 1998 May;2(5):425-9.
A laboratory for routine culturing of Mycobacterium tuberculosis.
Investigation of an episode of laboratory cross contamination using IS6110 restriction fragment length polymorphism (RFLP) typing. Improvement of laboratory protocols to prevent contaminations in the future. To stress the importance of 'good laboratory practice', and interaction with clinicians about laboratory results.
Fingerprinting of mycobacterial isolates from 1) cultures suspected of being contaminated and 2) strains suspected of being the source of the cross-contamination.
RFLP typing results indicated that clinical samples were contaminated by strains which had been processed in species identification procedures one day earlier in the same safety cabinet. This cross contamination also resulted in exceptional RFLP typing results--mixed banding patterns. Three patients were treated on the basis of false-positive laboratory results. Because the laboratory results were confusing for the clinicians, the treatment of one true tuberculosis patient was severely delayed.
'Good laboratory practice' is very important to prevent cross contamination. RFLP typing proved to be a useful tool to trace the source of contamination. Interaction with clinicians receiving doubtful results is of the utmost importance.
一个用于结核分枝杆菌常规培养的实验室。
运用IS6110限制性片段长度多态性（RFLP）分型法调查一起实验室交叉污染事件。改进实验室操作规程以防止未来发生污染。强调“良好实验室规范”的重要性，以及与临床医生就实验室结果进行沟通。
对以下两类分枝杆菌分离株进行指纹图谱分析：1）疑似受到污染的培养物；2）疑似为交叉污染源头的菌株。
RFLP分型结果表明，临床样本被前一天在同一安全柜中进行菌种鉴定程序时处理过的菌株污染。这种交叉污染还导致了异常的RFLP分型结果——混合条带模式。三名患者基于假阳性实验室结果接受了治疗。由于实验室结果让临床医生感到困惑，一名真正的结核病患者的治疗被严重延误。
“良好实验室规范”对于防止交叉污染非常重要。RFLP分型被证明是追踪污染源头的有用工具。与收到可疑结果的临床医生进行沟通至关重要。
