Olmos-Zúñiga J Raúl, Jasso-Victoria Rogelio, Sotres-Vega Avelina, Gaxiola-Gaxiola Miguel O, Argote-Green Luis M, Lara-Guerra Humberto, Santillán-Doherty Patricio
Departamento de Investigación en Cirugía Experimental, Instituto Nacional de Ciencias Médicas y de la Nutrición Salvador Zubirán.
Rev Invest Clin. 2002 Jul-Aug;54(4):334-41.
In this study we assessed the usefulness, healing, as well as the integration to lung tissue of glutaraldehyde preserved at 0.5% bovine pericardium GPBP and lyophilized (GPBPL), after reinforced resection of lung tissue in dogs by thoracotomy or thoracoscopy.
GPBP and GPBPL were prepared and used to reinforce the suture line of lung resection in 30 mongrel dogs: Group I (n = 6): The GPBP were fixed on the lung with 4-0 polypropylene by thoracotomy. Group II (n = 6): The resection and fixed of the GPBP were performed with an linear stapler by thoracotomy. Group III (n = 6): The resection and fixed of the GPBPL were performed with an linear stapler by thoracotomy. Group IV (n = 6): The resection and fixed of the GPBP strips were performed with a linear stapler by thoracoscopy. Group V: The resection and fixed of the GPBPL strips were performed with a linear stapler by thoracoscopy. Clinico-radiological evaluation was done until euthanasia of all animals at week 8 postop. Progressive insufflation up to 40 cm H2O of airway pressure was done to evaluated resistance of the heal in the suture line reinforced. Macroscopic, and microscopic examination of the GPBP, GPBPL and lung were evaluated.
All animals survived the surgical procedure and study time (8 weeks). No airleaks were evident at any time during the study including the insufflation test. Macroscopic examination of the GPBP and GPBPL showed good adaptation to the lung tissue. Microscopically all animals presented good healing with deposition of fibrotic tissue layer on the GPBP and GPBPL.
GPBP and GPBPL are an adequate materials to reinforce lung staple line, when resection of lung tissue was performed in dogs by thoracotomy or thoracoscopy.
在本研究中，我们评估了25%戊二醛保存的0.5%牛心包（GPBP）和冻干牛心包（GPBPL）在通过开胸或胸腔镜对犬肺组织进行强化切除术后，其对肺组织的实用性、愈合情况以及整合情况。
制备GPBP和GPBPL，并用于强化30只杂种犬肺切除的缝合线：第一组（n = 6）：通过开胸用4-0聚丙烯将GPBP固定于肺。第二组（n = 6）：通过开胸用线性缝合器进行GPBP的切除和固定。第三组（n = 6）：通过开胸用线性缝合器进行GPBPL的切除和固定。第四组（n = 6）：通过胸腔镜用线性缝合器进行GPBP条带的切除和固定。第五组：通过胸腔镜用线性缝合器进行GPBPL条带的切除和固定。在术后第8周对所有动物实施安乐死之前进行临床放射学评估。逐步增加气道压力至40 cm H2O以评估强化缝合线处愈合的阻力。对GPBP、GPBPL和肺进行宏观和微观检查。
所有动物均存活至手术过程及研究结束（8周）。在研究期间包括通气试验的任何时候均未发现明显漏气。GPBP和GPBPL的宏观检查显示与肺组织适应性良好。显微镜下，所有动物均愈合良好，在GPBP和GPBPL上有纤维化组织层沉积。
当通过开胸或胸腔镜对犬进行肺组织切除时，GPBP和GPBPL是强化肺缝合线的合适材料。
