Santillan-Doherty P, Jasso-Victoria R, Sotres-Vega A, Olmos R, Arreola J L, Garcia D, Vanda B, Gaxiola M, Santibañez A, Martin S, Cabello R
Department of Experimental Surgery, National Institute of Respiratory Diseases, Tlalpan, Mexico.
J Invest Surg. 1996 Jan-Feb;9(1):45-55. doi: 10.3109/08941939609012459.
Glutaraldehyde-preserved bovine pericardium (GPBP) is evaluated as a bioprosthesis for the reconstruction of surgical defects in the thoracoabdominal wall. The mechanical properties of bovine pericardium preserved at different concentrations of glutaraldehyde were studied. Samples preserved in 0.5% glutaraldehyde showed a significantly higher tensile strength (11.7 +/- 0.8 N/mm2) than samples preserved in 2.5, 5, or 10% (similar to pericardium preserved in normal saline). The percentage of elongation was significantly lower than samples preserved in 1, 2.5, and 5% glutaraldehyde. GPBP at 0.5% was used to repair experimentally induced defects of the abdominal wall (n = 9), chest wall (n = 6), diaphragm (n = 6), and sternum (n = 7). All animals presented adequate tolerance to the material used and no case of infection or rejection of the material was seen in any of the animals. Finally, 0.5% GPBP was used clinically in a series of 40 patients: postincisional abdominal hernia (n = 30), inguinal hernia (n = 8), diaphragmatic hernia (n = 1), and congenital pelvic defect with prolapse of abdominal organs (n = 1). Surgical use showed that GPBP was a very manageable material and long-term results were good in 37 patients with a mean follow up of 18 months (range 5-35 months). Six patients presented seroma formation (all abdominal hernia patients), three of which eventually developed infection and had the GPBP patch removed at 3, 5, and 7 months postoperatively. The rest of the patients presented good scar formation with adequate resistance at the area of implantation. GPBP is a biological material with sufficient resistance to be used surgically in the repair of thoracoabdominal defects. Ideal concentration of glutaraldehyde to be used in the preparation-preservation of the material is 0.5% since higher concentration negatively affect its tensile rupture strength and elongation.
戊二醛保存的牛心包（GPBP）被评估为一种用于修复胸腹壁手术缺损的生物假体。研究了保存在不同浓度戊二醛中的牛心包的力学性能。保存在0.5%戊二醛中的样本显示出比保存在2.5%、5%或10%（类似于保存在生理盐水中的心包）中的样本显著更高的拉伸强度（11.7±0.8 N/mm2）。伸长率百分比显著低于保存在1%、2.5%和5%戊二醛中的样本。0.5%的GPBP被用于修复实验诱导的腹壁缺损（n = 9）、胸壁缺损（n = 6）、膈肌缺损（n = 6）和胸骨缺损（n = 7）。所有动物对所用材料均有足够的耐受性，且在任何动物中均未观察到材料感染或排斥的情况。最后，0.5%的GPBP在40例患者中进行了临床应用：切口后腹疝（n = 30）、腹股沟疝（n = 8）、膈肌疝（n = 1）和伴有腹部器官脱垂的先天性盆腔缺损（n = 1）。手术应用表明，GPBP是一种非常易于操作的材料，37例患者长期效果良好，平均随访18个月（范围5 - 35个月）。6例患者出现血清肿形成（均为腹疝患者），其中3例最终发生感染，并在术后3、5和7个月移除了GPBP补片。其余患者在植入区域形成了良好的瘢痕，具有足够的抵抗力。GPBP是一种具有足够抵抗力的生物材料，可用于手术修复胸腹部缺损。用于材料制备保存的戊二醛理想浓度为0.5%，因为更高的浓度会对其拉伸断裂强度和伸长率产生负面影响。
