Mace Emma S, Godwin Ian D
M.S. Swaminathan Applied Genomics Laboratory, International Crops Research Institute for the Semi-Arid Tropics, Patancheru, Andhra Pradesh, India.
Genome. 2002 Oct;45(5):823-32. doi: 10.1139/g02-045.
Microsatellite-containing sequences were isolated from enriched genomic libraries of taro (Colocasia esculenta (L.) Schott). The sequencing of 269 clones yielded 77 inserts containing repeat motifs. The majority of these (81.7%) were dinucleotide or trinucleotide repeats. The GT/CA repeat motif was the most common, accounting for 42% of all repeat types. From a total of 43 primer pairs designed, 41 produced markers within the expected size range. Sixteen (39%) were polymorphic when screened against a restricted set of taro genotypes from Southeast Asia and Oceania, with an average of 3.2 alleles detected on each locus. These markers represent a useful resource for taro germplasm management, genome mapping, and marker-assisted selection.
从芋头（Colocasia esculenta (L.) Schott）的富集基因组文库中分离出含有微卫星的序列。对269个克隆进行测序，得到77个含有重复基序的插入片段。其中大多数（81.7%）是二核苷酸或三核苷酸重复序列。GT/CA重复基序最为常见，占所有重复类型的42%。在总共设计的43对引物中，41对产生了预期大小范围内的标记。当针对来自东南亚和大洋洲的一组有限的芋头基因型进行筛选时，16个（39%）具有多态性，每个位点平均检测到3.2个等位基因。这些标记是芋头种质管理、基因组作图和标记辅助选择的有用资源。
