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芋(Colocasia esculenta)11 个新微卫星位点的特征。

Characterization of 11 new microsatellite loci in taro (Colocasia esculenta).

机构信息

Key Laboratory of MOE for Plant Development Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China Wuhan National Field Observation and Research Station for Aquatic Vegetables, Wuhan 430065, China.

出版信息

Mol Ecol Resour. 2009 Mar;9(2):582-4. doi: 10.1111/j.1755-0998.2008.02441.x. Epub 2009 Jan 31.

Abstract

Eleven new microsatellite markers were isolated from taro, Colocasia esculenta (L.) Schott, a root crop widely distributed all over the world. Forty-eight primer pairs were designed from a microsatellite-enriched genomic library, of which 11 primer pairs have polymorphisms in 30 individuals tested from a population in China, which revealed two to six alleles per locus with the observed and expected heterozygosity levels ranging from 0 to 0.733 and from 0.381 to 0.731, respectively. These new genetic markers will be useful for the study of taro germplasm management and population evolution in the future.

摘要

从分布于世界各地的根茎作物芋艿(Colocasia esculenta (L.) Schott)中分离出 11 个新的微卫星标记。从微卫星富集基因组文库中设计了 48 对引物,其中 11 对引物在来自中国种群的 30 个个体中具有多态性,每个位点显示 2 到 6 个等位基因,观测到的和预期的杂合度水平分别为 0 到 0.733 和 0.381 到 0.731。这些新的遗传标记将来可用于芋艿种质资源管理和种群进化的研究。

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