Hoshino Sachiko, Ohkoshi Norio, Ishii Akiko, Shoji Shinichi
Department of Neurology, Institute of Clinical Medicine, University of Tsukuba, Japan.
J Muscle Res Cell Motil. 2002;23(2):131-8. doi: 10.1023/a:1020256316659.
The expression of alpha-dystrobrevin and dystrophin in rat tibialis anterior muscles was chronologically evaluated during a cycle of regeneration after myonecrosis induced by the injection of cardiotoxin. In immunohistochemical studies, alpha-dystrobrevin and dystrophin were first stained weakly at the sarcolemma of some regenerating muscle fibers on day 5. On day 7, alpha-dystrobrevin was still stained weakly, whereas dystrophin was stained conspicuously. After day 10, alpha-dystrobrevin and dystrophin were both stained conspicuously on almost all regenerating muscle fibers. In the Western blot analysis, alpha-dystrobrevin and dystrophin were first detected as visible bands on days 5 and 7, respectively. The bands of alpha-dystrobrevin and dystrophin both darkened sequentially up to day 10. The protein levels based on the densitometrical analysis of the bands on each day were converted to the percentage of the protein level on day 28, which was taken as 100%. The sequential line based on these data showed that alpha-dystrobrevin and dystrophin reached 50% of the protein level on day 28 by 6.6 and 5.3 days, respectively. These data provide evidence that alpha-dystrobrevin regenerates more slowly than dystrophin in skeletal muscle.
在注射心脏毒素诱导大鼠胫骨前肌发生肌坏死之后的再生周期中，按时间顺序评估了α-肌营养不良蛋白短链和肌营养不良蛋白在大鼠胫骨前肌中的表达情况。在免疫组织化学研究中，第5天时，α-肌营养不良蛋白短链和肌营养不良蛋白在一些再生肌纤维的肌膜上首次出现弱阳性染色。第7天时，α-肌营养不良蛋白短链仍为弱阳性染色，而肌营养不良蛋白染色明显。第10天之后，α-肌营养不良蛋白短链和肌营养不良蛋白在几乎所有再生肌纤维上均染色明显。在蛋白质印迹分析中，α-肌营养不良蛋白短链和肌营养不良蛋白分别在第5天和第7天首次检测到可见条带。直至第10天，α-肌营养不良蛋白短链和肌营养不良蛋白的条带均依次加深。根据每天条带的光密度分析得出的蛋白质水平换算为第28天蛋白质水平的百分比，第28天的蛋白质水平被视为100%。基于这些数据的序列线显示，α-肌营养不良蛋白短链和肌营养不良蛋白分别在6.6天和5.3天达到第28天蛋白质水平的50%。这些数据证明，在骨骼肌中α-肌营养不良蛋白短链的再生速度比肌营养不良蛋白慢。
