胆固醇对DBA/1J小鼠II型胶原诱导性关节炎影响的评估：一项放射自显影研究。
Evaluation of the cholesterol influence in type II collagen-induced arthritis in DBA/1J mice: an autoradiographic study.
作者信息
Hamer Erica R, Apfel M I R, Carvalho J J, Pereira M J S, Levy R A
机构信息
Department of Biophysics and Biometrics, IBRAG, Universidade do Estado do Rio de Janeiro, Brasil.
出版信息
J Cell Mol Med. 2002 Jul-Sep;6(3):407-14. doi: 10.1111/j.1582-4934.2002.tb00519.x.
In order to verify the cholesterol influence in RA severity in DBA/1J mice, we quantified the cholesterol present in the knee joints of normal (N) and with collagen II induced arthritis (CIA). Forty male DBA/1J mice, were divided in normal (n=20) and CIA group (n=20). Mice in CIA group were injected with 100 microg of collagen II emulsified in Freund's complete adjuvant. Sixteen DBA/1J (8 N and 8 CIA) received an injection of 2.96 x 10(6) Bq of (3)H-cholesterol and were anesthetized and sacrificed. Semi-fine sections were covered with LM-1 emulsion, exposed for six weeks and developed. Collagen induced edema, erythema and dysfunction of knee joints in CIA group. Radioactive cholesterol was located more on the synovial membrane, where we found the greatest density of silver grains, significantly (P<0.0001) higher in group CIA vs. controls (61-/+2.3 X 18-/+0.7). We conclude that the cholesterol deposits on the synovial membrane is related to CIA severity.
为了验证胆固醇对DBA/1J小鼠类风湿性关节炎（RA）严重程度的影响，我们对正常（N）小鼠和胶原II诱导性关节炎（CIA）小鼠膝关节中的胆固醇进行了定量分析。40只雄性DBA/1J小鼠被分为正常组（n = 20）和CIA组（n = 20）。CIA组小鼠注射了100微克在弗氏完全佐剂中乳化的胶原II。16只DBA/1J小鼠（8只正常小鼠和8只CIA小鼠）接受了2.96 x 10(6) Bq的(3)H - 胆固醇注射，然后麻醉并处死。半薄切片用LM - 1乳剂覆盖，曝光六周后显影。CIA组出现胶原诱导的膝关节水肿、红斑和功能障碍。放射性胆固醇更多地位于滑膜上，在滑膜处我们发现银颗粒密度最高，CIA组与对照组相比显著更高（P < 0.0001）（61 -/+2.3 X 18 -/+0.7）。我们得出结论，滑膜上的胆固醇沉积与CIA的严重程度有关。
Zotero 插件