放射性药物在血液成分上生物分布的体外放射自显影研究。

In vitro radioautographic studies of the biodistribution of radiopharmaceuticals on blood elements.

作者信息

Ripoll-Hamer E, De-Paula E F, Freitas L C, Pereira M J, Fonseca L M, Gutfilen B, Carvalho J J, Pôrto L C, Bernardo-Filho M

机构信息

Centro de Pesquisa Básica, Instituto Nacional de Câncer, Rio de Janeiro, Brasil.

出版信息

Braz J Med Biol Res. 1998 Feb;31(2):303-6. doi: 10.1590/s0100-879x1998000200014.

DOI:10.1590/s0100-879x1998000200014

PMID:9686153

Abstract

In the present study we evaluated the binding of the radiopharmaceuticals sodium pertechnetate (Na 99mTcO4), methylenediphosphonic acid (99mTc-MDP) and glucoheptonate acid (99mTc-GHA) to blood elements using centrifugation and radioautographic techniques. Heparinized blood was incubated with the labelled compounds for 0, 1, 2, 3, 4, 6 and 24 h. Plasma (P) and blood cells (BC) were isolated and precipitated with 5% trichloroacetic acid (TCA), and soluble (SF) and insoluble fractions (IF) were separated. Blood samples were prepared (0 and 24 h) and coated with LM-1 radioautographic emulsions and percent radioactivity (%rad) in P and BC was determined. The binding of Na 99mTcO4 (%rad) to P was 61.2% (0 h) and 46.0% (24 h), and radioautography showed 63.7% (0 h) and 43.3% (24 h). The binding to BC was 38.8% (0 h) and 54.0% (24 h), and radioautography showed 36.3% (0 h) and 56.7% (24 h). 99mTc-MDP study presented 91.1% (0 h) to P and 87.2% (24 h), and radioautography showed 67.9% (0 h) and 67.4% (24 h). The binding to BC was 8.9% (0 h) and 12.8% (24 h), and radioautography showed 32.1% (0 h) and 32.6% (24 h). 99mTc-GHA study was 90.1% (0 h) to P and 79.9% (24 h), and radioautography showed 67.2% (0 h) and 60.1% (24 h). The binding to BC was 9.9% (0 h) and 20.1% (24 h), and radioautography showed 32.8% (0 h) and 39.9% (24 h). The comparison of the obtained results suggests that the binding to plasma and blood cells in the two techniques used (radioautography and centrifugation) is qualitatively in accordance.

摘要

在本研究中，我们使用离心和放射自显影技术评估了放射性药物高锝酸钠（Na 99mTcO4）、亚甲基二膦酸（99mTc-MDP）和葡庚糖酸盐（99mTc-GHA）与血液成分的结合情况。将肝素化血液与标记化合物孵育0、1、2、3、4、6和24小时。分离血浆（P）和血细胞（BC），并用5%三氯乙酸（TCA）沉淀，然后分离可溶性（SF）和不溶性部分（IF）。制备血液样本（0和24小时），并用LM-1放射自显影乳剂包被，测定P和BC中的放射性百分比（%rad）。Na 99mTcO4与P的结合（%rad）在0小时为61.2%，24小时为46.0%，放射自显影显示0小时为63.7%，24小时为43.3%。与BC的结合在0小时为38.8%，24小时为54.0%，放射自显影显示0小时为36.3%，24小时为56.7%。99mTc-MDP研究显示与P的结合在0小时为91.1%，24小时为87.2%，放射自显影显示0小时为67.9%，24小时为67.4%。与BC的结合在0小时为8.9%，24小时为12.8%，放射自显影显示0小时为32.1%，24小时为32.6%。99mTc-GHA研究显示与P的结合在0小时为90.1%，24小时为79.9%，放射自显影显示0小时为67.2%，24小时为60.1%。与BC的结合在0小时为9.9%，24小时为20.1%，放射自显影显示0小时为32.8%，24小时为39.9%。对所得结果的比较表明，在所用的两种技术（放射自显影和离心）中，与血浆和血细胞的结合在定性上是一致的。