Deng Lianfu, Feng Wei, Zhang Yue, Zhu Yaping
Shanghai Institute of Traumatology and Orthopaedics, Shanghai 200025, China.
Zhonghua Wai Ke Za Zhi. 2002 Aug;40(8):592-5.
To investigate the probabilities of core-biding factor a1 (Cbfa1) expression by human skin fibroblasts induced in vitro.
The fibroblasts were isolated, purified from human skin, and were grown in incubation in the media of TNF-alpha, BMP-2, and combined TNF-alpha and BMP-2 at certain concentrations, respectively. The changes in biological features of these fibroblasts correlated with osteogenesis were detected by immunohistochemistry and RT-PCR assay.
TNF-alpha could switch phenotype of collagen in fibroblasts from Type I and III to Type I and induce fibroblasts to express Ras and BMP type I receptor (BMPR-IA). TNF-alpha in combination with BMP-2 could induce fibroblasts to express Cbfa1 and osteocalcin mRNA.
Human skin fibroblast could be induced into pro-osteoblast expressing Cbfa1, an osteoblast-specific transcription factor and a regulation of osteoblast differentiation, and combined use of TNF-alpha and BMP-2 was one of the regulating factors.
研究体外诱导人皮肤成纤维细胞核心结合因子a1（Cbfa1）表达的可能性。
从人皮肤中分离、纯化成纤维细胞，分别在含有一定浓度肿瘤坏死因子-α（TNF-α）、骨形态发生蛋白-2（BMP-2）以及TNF-α与BMP-2组合的培养基中培养。通过免疫组织化学和逆转录-聚合酶链反应（RT-PCR）检测这些与成骨相关的成纤维细胞生物学特性的变化。
TNF-α可使成纤维细胞中胶原蛋白的表型从I型和III型转变为I型，并诱导成纤维细胞表达Ras和I型骨形态发生蛋白受体（BMPR-IA）。TNF-α与BMP-2联合使用可诱导成纤维细胞表达Cbfa1和骨钙素mRNA。
人皮肤成纤维细胞可被诱导成为表达Cbfa1的成骨前体细胞，Cbfa1是一种成骨细胞特异性转录因子，对成骨细胞分化起调节作用，TNF-α与BMP-2联合使用是调节因子之一。
