McKenna F, McLaughlin P J, Lewis B J, Sibbring G C, Cummerson J A, Bowen-Jones D, Moots R J
Department of Immunology, University of Liverpool, Royal Liverpool University Hospital, Daulby Street, Liverpool L69 3GA, UK.
J Neuroimmunol. 2002 Nov;132(1-2):34-40. doi: 10.1016/s0165-5728(02)00280-1.
This study documents expression of dopamine (DA) receptors on leukocyte subpopulations using flow cytometric techniques to identify dopamine receptors with subtype-specific antibodies. Of the D1-like receptor family (D(1) and D(5)), only D(5) was detected, and of the D2-like receptor family (D(2), D(3) and D(4)), all dopamine receptors were detected. T-lymphocytes and monocytes had low expression of dopamine receptors, whereas neutrophils and eosinophils had moderate expression. B cells and NK cells had higher and more consistent expression. Dopamine receptors D(3) and D(5) were found in most individuals whereas D(2) and D(4) had more variable expression. D(1) was never found.
本研究利用流式细胞术，通过亚型特异性抗体鉴定多巴胺（DA）受体，记录白细胞亚群上多巴胺受体的表达情况。在D1样受体家族（D(1)和D(5)）中，仅检测到D(5)；在D2样受体家族（D(2)、D(3)和D(4)）中，所有多巴胺受体均被检测到。T淋巴细胞和单核细胞的多巴胺受体表达较低，而中性粒细胞和嗜酸性粒细胞有中度表达。B细胞和NK细胞有更高且更一致的表达。大多数个体中可检测到多巴胺受体D(3)和D(5)，而D(2)和D(4)的表达更具变异性。从未检测到D(1)。
