Helfrich Marcus R, Mangeney-Slavin Lauren K, Long M Scott, Djoko Karrera Y, Keating Christine D
Department of Chemistry, The Pennsylvania State University, University Park 16802, USA.
J Am Chem Soc. 2002 Nov 13;124(45):13374-5. doi: 10.1021/ja028157+.
We report the synthesis and initial characterization of approximately 10 mum diameter lipid vesicles that contain two distinct aqueous phases. The aqueous two-phase system is a dextran/poly(ethylene glycol) solution that exhibits temperature-dependent phase behavior. Vesicles were prepared above the phase transition temperature of the polymer solution. Upon cooling to room temperature, the polymer solution phase separated both within the vesicles and in the bulk solution. The location of poly(ethylene glycol)-rich and dextran-rich phases was determined by fluorescence microscopy. These structures are exciting in that they enable for the first time the interior volume of liposomes to be structured.
我们报道了直径约10微米、含有两个不同水相的脂质囊泡的合成及初步表征。水两相体系是一种葡聚糖/聚乙二醇溶液，其呈现出温度依赖性相行为。囊泡在聚合物溶液的相转变温度以上制备。冷却至室温后，聚合物溶液在囊泡内和本体溶液中均发生相分离。通过荧光显微镜确定了富含聚乙二醇和富含葡聚糖相的位置。这些结构令人兴奋，因为它们首次使脂质体的内部体积能够被构建。
