Liou Shiow-Wen, Chiu Cheng-Jen, Wang I-Jong
Department of Ophthalmology, Taipei Women's and Children's Hospital, Taipei, Taiwan.
J Ocul Pharmacol Ther. 2002 Oct;18(5):469-73. doi: 10.1089/10807680260362740.
Epinephrine is frequently used in the phacoemulsification to dilate pupils. To determine the effects of different concentration of epinephrine on the corneal endothelial cells, twenty-eight rabbit eyes were equally divided into four groups. Solutions, which contained normal saline, 1:1000 epinephrine, 1:5000 epinephrine and 1:10000 epinephrine respectively, were injected into the anterior chambers of the eyes of four groups of rabbits. In vivo morphological changes of corneal endothelium and changes of thickness were checked with specular microscopy. In vitro morphological evaluation of corneal endothelium was observed in excised corneal buttons stained with alizarin red with trypan blue, and with scanning electron microscopy. Our results showed that there was no significant difference in cell density and corneal thickness among the four groups. Alizarin red with trypan blue stain and SEM exam revealed smooth and distinct cell borders of endothelial cells in each group. Intracameral injection of epinephrine does not produce toxic effect on corneal endothelial cells in rabbits.
肾上腺素常用于白内障超声乳化术中散瞳。为确定不同浓度肾上腺素对兔角膜内皮细胞的影响，将28只兔眼平均分为四组。分别向四组兔眼的前房内注入含有生理盐水、1:1000肾上腺素、1:5000肾上腺素和1:10000肾上腺素的溶液。用角膜内皮镜检查角膜内皮的活体形态变化及厚度变化。用茜素红-台盼蓝染色法对切除的角膜片进行体外角膜内皮形态学评估，并进行扫描电子显微镜检查。结果显示，四组之间的细胞密度和角膜厚度无显著差异。茜素红-台盼蓝染色和扫描电子显微镜检查显示，每组内皮细胞的边界光滑且清晰。前房内注射肾上腺素对兔角膜内皮细胞无毒性作用。
