Kim Su-Young, Park Young-Hoon, Lee Young-Chun
Department of Ophthalmology and Visual Science, College of Medicine, The Catholic University of Korea, Seoul, Korea.
Clin Exp Ophthalmol. 2008 May;36(4):367-70. doi: 10.1111/j.1442-9071.2008.01771.x.
To compare the effect on corneal endothelial cells among intracameral moxifloxacin, levofloxacin and cefazoline in rabbits.
Thirty-six eyes from eighteen rabbits were divided into four groups. The control solution (balanced salt solution), cefazolin 1000 microg/0.1 mL, levofloxain 500 microg/0.1 mL or moxifloxacin 500 microg/0.1 mL was injected into the anterior chamber of the rabbit eye. The central corneal thickness was assessed before the injection of intracameral antibiotics. Endothelial toxicity was evaluated according to the central corneal thickness, specular microscopy, scanning electron microscopy and transmission electron microscopy 1 day after injection.
Before the injection, the central corneal thickness was not different among four groups. After injection, there was no significant difference of the corneal thickness and endothelial cell count between the control and study groups. The central corneal thickness after injection of antibiotics did not significantly change when compared with control. Scanning electron microscopy showed normal hexagonality. Normal architecture of the endothelial cells was noted in transmission electron microscopy in all four groups.
Intracameral injection of antibiotics (cefazolin 1000 microg/0.1 mL, levofloxain 500 microg/0.1 mL, moxifloxacin 500 microg/0.1 mL) did not show significant toxicity on the endothelial cells compared with the control group. Intracameral injection of one of these antibiotics appears to be safe for surgical prophylaxis.
比较莫西沙星、左氧氟沙星和头孢唑林前房内注射对兔角膜内皮细胞的影响。
将18只兔的36只眼分为四组。将对照溶液（平衡盐溶液）、1000微克/0.1毫升头孢唑林、500微克/0.1毫升左氧氟沙星或500微克/0.1毫升莫西沙星注入兔眼前房。在前房注射抗生素前评估中央角膜厚度。注射后1天，根据中央角膜厚度、镜面显微镜检查、扫描电子显微镜检查和透射电子显微镜检查评估内皮毒性。
注射前，四组中央角膜厚度无差异。注射后，对照组与研究组之间的角膜厚度和内皮细胞计数无显著差异。与对照组相比，注射抗生素后的中央角膜厚度无显著变化。扫描电子显微镜显示正常六边形。所有四组透射电子显微镜检查均显示内皮细胞结构正常。
与对照组相比，前房内注射抗生素（1000微克/0.1毫升头孢唑林、500微克/0.1毫升左氧氟沙星、500微克/0.1毫升莫西沙星）对内皮细胞未显示出显著毒性。前房内注射这些抗生素中的一种似乎对手术预防是安全的。
