Hong Ji Wook, Park Jin Hyoung, Kim Eun Soon, Kim Jae Yong, Kim Myoung Joon, Tchah Hungwon
*Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea; and †Research Institute for Biomacromolecules, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Republic of Korea.
Cornea. 2015 Apr;34(4):460-3. doi: 10.1097/ICO.0000000000000312.
The aim of this study was to investigate the effect of intracameral injection of bisulfite-containing phenylephrine on rabbit corneal endothelium cells in rabbits.
Fifteen eyes of 15 New Zealand white rabbits were divided into 3 groups. Five eyes from each group were injected with 0.5% (0.5 mL, 0.05% bisulfite, group 1), 1.0% bisulfite-containing phenylephrine (0.5 mL, 0.1% bisulfite, group 2), or an equal volume of balanced salt solution as a control (group 3). Specular microscopy, corneal pachymetry, and a slit-lamp examination were used to evaluate corneal endothelial cell loss and other complications before injection, and at 1 hour and 1 week after injection. For morphological studies, corneal buttons were excised at 1 week and stained with alizarin red and trypan blue. Optical microscopy was used to examine 1 cornea from each of the 3 groups.
Within each group, there were no changes in specular microscopy and corneal pachymetry findings over the 1-week duration of the study. Although all groups showed the presence of several giant cells in the endothelium, the hexagonal shape of the corneal endothelium was morphologically preserved, and no abnormal endothelial cells were observed.
Intracameral injection of bisulfite-containing phenylephrine (up to 1.0%) does not affect endothelial cell viability or morphology of the rabbit cornea.
本研究旨在探讨前房内注射含亚硫酸氢盐的去氧肾上腺素对兔角膜内皮细胞的影响。
将15只新西兰白兔的15只眼分为3组。每组5只眼分别注射0.5%（0.5 mL，含0.05%亚硫酸氢盐，第1组）、1.0%含亚硫酸氢盐的去氧肾上腺素（0.5 mL，含0.1%亚硫酸氢盐，第2组），或等体积的平衡盐溶液作为对照（第3组）。在注射前、注射后1小时和1周，使用角膜内皮显微镜、角膜测厚仪和裂隙灯检查来评估角膜内皮细胞损失及其他并发症。为进行形态学研究，在1周时切除角膜纽扣并用地衣红和台盼蓝染色。使用光学显微镜检查3组中每组的1只角膜。
在研究的1周期间内，每组内角膜内皮显微镜和角膜测厚仪检查结果均无变化。尽管所有组的内皮中均可见多个巨大细胞，但角膜内皮的六边形形态在形态学上得以保留，且未观察到异常内皮细胞。
前房内注射含亚硫酸氢盐的去氧肾上腺素（浓度高达1.0%）不会影响兔角膜内皮细胞的活力或形态。
