Chitikila Carmelata, Huisinga Kathryn L, Irvin Jordan D, Basehoar Andrew D, Pugh B Franklin
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16803, USA.
Mol Cell. 2002 Oct;10(4):871-82. doi: 10.1016/s1097-2765(02)00683-4.
The TATA binding protein (TBP) is required for the expression of nearly all genes and is highly regulated both positively and negatively. Here, we use DNA microarrays to explore the genome-wide interplay of several TBP-interacting inhibitors in the yeast Saccharomyces cerevisiae. Our findings suggest the following: The NC2 inhibitor turns down, but not off, highly active genes. Autoinhibition of TBP through dimerization contributes to transcriptional repression, even at repressive subtelomeric regions. The TAND domain of TAF1 plays a primary inhibitory role at very few genes, but its function becomes widespread when other TBP interactions are compromised. These findings reveal that transcriptional output is limited in part by a collaboration of different combinations of TBP inhibitory mechanisms.
TATA结合蛋白（TBP）是几乎所有基因表达所必需的，并且受到正调控和负调控。在这里，我们使用DNA微阵列来探索酿酒酵母中几种与TBP相互作用的抑制剂在全基因组范围内的相互作用。我们的研究结果表明：NC2抑制剂会降低但不会关闭高活性基因的表达。TBP通过二聚化实现的自身抑制作用有助于转录抑制，即使在抑制性的亚端粒区域也是如此。TAF1的TAND结构域在极少数基因中起主要抑制作用，但当其他TBP相互作用受损时，其功能会变得普遍。这些发现揭示了转录输出在一定程度上受到TBP抑制机制不同组合协同作用的限制。
