Islam M R, Johne R, Raue R, Todd D, Müller H
Institute for Virology, Faculty of Veterinary Medicine, University of Leipzig, Germany.
J Vet Med B Infect Dis Vet Public Health. 2002 Sep;49(7):332-7. doi: 10.1046/j.1439-0450.2002.00581.x.
Chicken anaemia virus (CAV) was detected in the bursa of Fabricius of a 4-week-old chicken obtained from an outbreak of acute infectious bursal disease in Bangladesh. Repeated attempts to grow this virus in MDCC-MSB1 cells were not successful. A full-length PCR amplicon of the genome of this strain, designated as BD-3 CAV, was cloned and sequenced. The complete nucleotide sequence and the deduced amino acid sequence were compared with those of 12 other CAV strains. The genetic analysis of the amino acid sequences of VP1 indicated the possible existence of genetic groups among CAV strains, as BD-3 CAV along with four other strains (CIA-1, L-028, Isolate 704 and TR-20) formed a distinct lineage. These strains have four signatory amino acids in VP1, such as 75I/T, 97L, 139Q and 144Q, out of which the latter two are located in a small hydrophilic peak.
在从孟加拉国一次急性传染性法氏囊病暴发中获取的一只4周龄鸡的法氏囊中检测到鸡贫血病毒（CAV）。多次尝试在MDCC - MSB1细胞中培养这种病毒均未成功。克隆并测序了该毒株基因组的全长PCR扩增子，该毒株命名为BD - 3 CAV。将其完整核苷酸序列和推导的氨基酸序列与其他12株CAV毒株进行了比较。VP1氨基酸序列的遗传分析表明CAV毒株中可能存在遗传组，因为BD - 3 CAV与其他四株毒株（CIA - 1、L - 028、分离株704和TR - 20）形成了一个独特的谱系。这些毒株在VP1中有四个标志性氨基酸，即75I/T、97L、139Q和144Q，其中后两个位于一个小的亲水性峰中。
