Krapez Uros, Barlic-Maganja Darja, Toplak Ivan, Hostnik Peter, Rojs Olga Zorman
Institute of Poultry Health, Veterinary Faculty, University of Ljubljana, Gerbikeva 60, 1000 Ljubljana, Slovenia.
Avian Dis. 2006 Mar;50(1):69-76. doi: 10.1637/7413.1.
The presence of chicken anemia virus (CAV) in Slovenia was confirmed by inoculation of 1-day-old chickens without antibodies against CAV and isolation of the virus on the Marek's disease chicken cell-MSB1 line and by polymerase chain reaction (PCR). Experimental inoculation of 1-day-old chickens resulted in lower hematocrit values, atrophy of the thymus, and atrophy of bone marrow. CAV was confirmed by PCR in the thymus, bone marrow, bursa of Fabricius, liver, spleen, ileocecal tonsils, duodenum, and proventriculus. The nucleotide sequence of the whole viral protein (VP)1 gene was determined by direct sequencing. Alignment of VP1 nucleotide sequences of Slovenian CAV isolates (CAV-69/00, CAV-469/01, and CAV-130/03) showed 99.4% to 99.9% homology. The VP1 nucleotide sequence alignment of Slovenian isolates with 19 other CAV strains demonstrated 94.4% to 99.4% homology. Slovenian isolates shared highest homology with the BD-3 isolate from Bangladesh. Alignment of the deduced VP1 amino acids showed that the Slovenian isolates shared 100% homology and had an amino acid sequence most similar to the BD-3 strain from Bangladesh (99.6%) and were 99.1% similar to the G6 strain from Japan and the L-028 strain from the United States. The Slovenian isolates were least similar (96.6%) to the 82-2 strain from Japan. A phylogeneric analysis on the basis of the alignment of the VP1 amino acids showed that CAV isolates used in the study formed three groups that indicated the possible existence of genetic groups among CAV strains. The CAV isolates were grouped together independent of their geographic origin and pathogenicity.
通过对1日龄无鸡贫血病毒（CAV）抗体的雏鸡进行接种，并在马立克氏病鸡细胞-MSB1系上分离病毒以及采用聚合酶链反应（PCR），证实斯洛文尼亚存在CAV。对1日龄雏鸡进行实验性接种导致血细胞比容值降低、胸腺萎缩和骨髓萎缩。通过PCR在胸腺、骨髓、法氏囊、肝脏、脾脏、回盲部扁桃体、十二指肠和腺胃中证实了CAV的存在。通过直接测序确定了整个病毒蛋白（VP）1基因的核苷酸序列。斯洛文尼亚CAV分离株（CAV-69/00、CAV-469/01和CAV-130/03）的VP1核苷酸序列比对显示同源性为99.4%至99.9%。斯洛文尼亚分离株与其他19种CAV毒株的VP1核苷酸序列比对显示同源性为94.4%至99.4%。斯洛文尼亚分离株与来自孟加拉国的BD-3分离株具有最高的同源性。推导的VP1氨基酸序列比对显示，斯洛文尼亚分离株具有100%的同源性，其氨基酸序列与来自孟加拉国的BD-3毒株最相似（99.6%），与来自日本的G6毒株和来自美国的L-028毒株的相似性为99.1%。斯洛文尼亚分离株与来自日本的82-2毒株最不相似（96.6%）。基于VP1氨基酸序列比对的系统发育分析表明，研究中使用的CAV分离株形成了三组，这表明CAV毒株中可能存在基因群。CAV分离株聚集在一起，与其地理来源和致病性无关。
