小檗碱的抗菌活性——阔叶十大功劳的一种成分。
Antimicrobial activity of berberine--a constituent of Mahonia aquifolium.
作者信息
Cernáková M, Kostálová D
机构信息
Department of Environmental Science, Faculty of Chemical Technology, Slovak University of Technology, 812 37 Bratislava, Slovakia.
出版信息
Folia Microbiol (Praha). 2002;47(4):375-8. doi: 10.1007/BF02818693.
The antimicrobial activity of the protoberberine alkaloid, berberine, isolated from Mahonia aquifolium, was evaluated against 17 microorganisms including two Gram-negative bacteria--Pseudomonas aeruginosa and Escherichia coli (both resistant and sensitive), two Gram-positive bacteria--Bacillus subtilis and Staphylococcus aureus, Zoogloea ramigera, six filamentous fungi--Penicilium chrysogenum, Aspergillus niger, Aureobasidium pullulans (black and white strain), Trichoderma viride (original green strain and brown mutant), Fusarium nivale, Mycrosporum gypseum and two yeasts--Candida albicans and Saccharomyces cerevisiae. The IC50, minimum inhibitory concentration (MIC), minimum microbicidal concentration (MMC) and minimum microbistatic concentration (MMS) varied considerably depending on the microorganism tested, the sensitivity decreasing as follows: S. aureus > P. aeruginosa S (sensitive) > E. coli S > P. aeruginosa R (resistant) > E. coli R > B. subtilis > Z. ramigera > C. albicans > S. cerevisiae > A. pullulans B (black) > A. pullulans W (white) > T. viride Br (brown) > M. gypseum > A. niger > F. nivale > P. chrysogenum > T. viride G (green).
对从阔叶十大功劳中分离得到的原小檗碱生物碱黄连素的抗菌活性进行了评估，测试对象包括17种微生物，其中有两种革兰氏阴性菌——铜绿假单胞菌和大肠杆菌（耐药型和敏感型），两种革兰氏阳性菌——枯草芽孢杆菌和金黄色葡萄球菌，生枝动胶菌，六种丝状真菌——产黄青霉、黑曲霉、出芽短梗霉（黑色和白色菌株）、绿色木霉（原始绿色菌株和棕色突变体）、雪腐镰刀菌、石膏样小孢子菌，以及两种酵母菌——白色念珠菌和酿酒酵母。半数抑制浓度（IC50）、最低抑菌浓度（MIC）、最低杀菌浓度（MMC）和最低抑菌浓度（MMS）因测试的微生物不同而有很大差异，敏感性降低顺序如下：金黄色葡萄球菌＞铜绿假单胞菌敏感型＞大肠杆菌敏感型＞铜绿假单胞菌耐药型＞大肠杆菌耐药型＞枯草芽孢杆菌＞生枝动胶菌＞白色念珠菌＞酿酒酵母＞出芽短梗霉黑色菌株＞出芽短梗霉白色菌株＞绿色木霉棕色突变体＞石膏样小孢子菌＞黑曲霉＞雪腐镰刀菌＞产黄青霉＞绿色木霉原始绿色菌株。
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