Basha S Ameer, Mishra R K, Jha R N, Pandey V B, Singh U P
Department of Mycology and Plant Pathology, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi 221 005, India.
Folia Microbiol (Praha). 2002;47(2):161-5. doi: 10.1007/BF02817675.
Berberine and (+/-)-bicuculline were isolated from roots and leaves, respectively, of Corydalis chaerophylla. Both were effective in vitro against spore germination of some plant pathogenic fungi (Alternaria brassicicola, A. brassicae, A. cheiranthi, A. melongenae, A. solani, Colletotrichum musae, C. falcatum, Curvularia penniseti, C. lunata, C. maculans, C. pallescens, Curvularia sp., Erysiphe pisi, E. cichoracearum, Erysiphe sp., Fusarium udum, Helminthosporium spiciferum, H. penniseti, H. frumentacei, Heterosporium sp., Oidium erysiphoides and Ustilago cynodontis). Berberine and (+/-)-bicuculline significantly inhibited spore germination of all the fungi at concentrations of 100-1000 ppm. Berberine was effective against all the fungi at all concentrations; most of the fungi did not germinate at 1000 ppm. H. penniseti conidia did not germinate at any concentration of (+/-)-bicuculline. U. cynodontis was the least sensitive fungus at lower concentrations but 800 ppm dose was highly effective.
小檗碱和（±）-荷包牡丹碱分别从碎叶紫堇的根和叶中分离得到。二者在体外均对一些植物病原真菌（芸苔链格孢、甘蓝链格孢、桂竹香链格孢、茄链格孢、茄链格孢、香蕉炭疽病菌、甘蔗凤梨病菌、黍弯孢、新月弯孢、黄斑弯孢、苍白弯孢、弯孢属、豌豆白粉菌、瓜白粉菌、白粉菌属、木豆镰刀菌、稻长蠕孢、黍长蠕孢、小麦长蠕孢、异孢霉属、白粉寄生孢和狗牙根黑粉菌）的孢子萌发有效。小檗碱和（±）-荷包牡丹碱在100 - 1000 ppm浓度下均能显著抑制所有真菌的孢子萌发。小檗碱在所有浓度下对所有真菌均有效；在1000 ppm时，大多数真菌不萌发。（±）-荷包牡丹碱的任何浓度均不能使黍长蠕孢分生孢子萌发。狗牙根黑粉菌在较低浓度下是最不敏感的真菌，但800 ppm剂量非常有效。
