迷走神经节后去神经支配对麻醉绵羊腮腺唾液蛋白质分泌的影响。

The effect of parasympathetic postganglionic denervation on parotid salivary protein secretion in anaesthetized sheep.

作者信息

Edwards A V, Titchen D A

机构信息

Physiological Laboratory, University of Cambridge, UK.

出版信息

Auton Neurosci. 2002 Sep 30;100(1-2):50-7. doi: 10.1016/s1566-0702(02)00148-0.

DOI:10.1016/s1566-0702(02)00148-0

PMID:12422960

Abstract

Effects of unilateral parasympathetic denervation of ovine parotid glands were examined in anaesthetized sheep 21-28 days after nerve section. Parasympathetic denervation reduced the mass of the ipsilateral gland while increasing that of the contralateral gland to the extent that total gland mass was greater than in sheep with normally innervated glands. The spontaneous secretion (8.8 +/- 1.1 microl min(-1) g gland(-1)) was significantly less from denervated than from innervated glands of normal control animals (26.0 +/- 2.7 microl min(-1) g gland(-1); P< 0.01) and contained more protein. Rates of flow, and the outputs of sodium and potassium, in response to sympathetic stimulation, were similar from normally innervated and chronically denervated glands, when allowance was made for the discrepancy in weights, whereas the output of protein was significantly enhanced following parasympathetic denervation (innervated--31.4 +/- 7.3 microg g gland(-1), denervated--83.4 +/- 26.6 microg g gland(-1); P< 0.05). Intra-arterial infusions of acetylcholine (130 pmol min(-1) kg(-1)) elicited a flow of parotid saliva, the protein content of which was significantly enhanced by prior parasympathetic denervation. Intra-arterial infusions of vasoactive intestinal peptide (VIP; 2.5 pmol min(-1) kg(-1)) produced a small but statistically significant (P< 0.05) increase in the flow of parotid saliva from the contralateral, innervated but not from denervated glands. It also caused a small increase in protein output, which was significantly enhanced by prior denervation. VIP had no synergistic effect on the parotid responses to acetylcholine. The results show that the parasympathetic innervation to the parotid gland of the sheep exerts important trophic effects on the gland. Interaction of adrenergic and cholinergic receptors makes an important contribution to stimulation of the secretion of protein and prior denervation potentiates the protein responses to both acetylcholine and VIP.

摘要

在神经切断后21 - 28天，对麻醉的绵羊进行单侧腮腺副交感神经去神经支配，观察其影响。副交感神经去神经支配使同侧腺体质量减少，而对侧腺体质量增加，以至于腺体总质量大于神经支配正常的绵羊。去神经支配腺体的自发分泌量（8.8±1.1微升·分钟⁻¹·克腺体⁻¹）显著低于正常对照动物的神经支配腺体（26.0±2.7微升·分钟⁻¹·克腺体⁻¹；P<0.01），且蛋白质含量更高。在考虑重量差异后，正常神经支配和长期去神经支配的腺体对交感神经刺激的流速以及钠和钾的输出相似，而副交感神经去神经支配后蛋白质输出显著增加（神经支配——31.4±7.3微克·克腺体⁻¹，去神经支配——83.4±26.6微克·克腺体⁻¹；P<0.05）。动脉内注射乙酰胆碱（130皮摩尔·分钟⁻¹·千克⁻¹）可引起腮腺唾液分泌，其蛋白质含量因先前的副交感神经去神经支配而显著增加。动脉内注射血管活性肠肽（VIP；2.5皮摩尔·分钟⁻¹·千克⁻¹）使对侧神经支配但未去神经支配的腺体的腮腺唾液流速有小幅但具有统计学意义（P<0.05）的增加。它还使蛋白质输出略有增加，先前的去神经支配使其显著增强。VIP对腮腺对乙酰胆碱的反应无协同作用。结果表明，绵羊腮腺的副交感神经支配对腺体发挥重要的营养作用。肾上腺素能和胆碱能受体的相互作用对蛋白质分泌的刺激起重要作用，先前的去神经支配增强了对乙酰胆碱和VIP的蛋白质反应。