[三七总皂苷与甲泼尼龙在大鼠肺纤维化模型中的作用机制及效果]
[The mechanisms and effects of panax notoginside and methylprednisolone in a rat model of pulmonary fibrosis].
作者信息
Li Xuejun, Cui Shehuai
机构信息
Department of Respiratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
出版信息
Zhonghua Jie He He Hu Xi Za Zhi. 2002 Sep;25(9):520-3.
OBJECTIVE
To study the effects of panax notoginside (PNS) and methylprednisolone (solu-medrol) on the development of pulmonary fibrosis and the possible underlining mechanisms.
METHODS
Rats were divided into 3 groups: pulmonary fibrosis group, PNS treated group and solu-medrol treated group. On days 1, 3, 7, 14, and 28 after bleomycin treatment, 5 rats in each group were killed and the lungs and plasma were harvested for histopathological studies, immunohistochemical determination of collagen I and III and ELISA detection of MIP-1alpha and MCP-1.
RESULTS
The degrees of fibrosis, the levels of collagen I and III, and the levels of MIP-1alpha and MCP-1 decreased significantly in the PNS and solu-medrol treated groups as compared with the fibrosis group (P < 0.05).
CONCLUSION
Both PNS and solu-medrol are effective in modulating the process of pulmonary fibrosis induced by bleomycin in rats.
目的
研究三七总皂苷（PNS）和甲泼尼龙（甲强龙）对肺纤维化发展的影响及其可能的潜在机制。
方法
将大鼠分为3组：肺纤维化组、PNS治疗组和甲泼尼龙治疗组。在博来霉素治疗后的第1、3、7、14和28天，每组处死5只大鼠，采集肺组织和血浆进行组织病理学研究、免疫组织化学法检测Ⅰ型和Ⅲ型胶原蛋白以及酶联免疫吸附测定法检测MIP-1α和MCP-1。
结果
与纤维化组相比，PNS治疗组和甲泼尼龙治疗组的纤维化程度、Ⅰ型和Ⅲ型胶原蛋白水平以及MIP-1α和MCP-1水平均显著降低（P < 0.05）。
结论
PNS和甲泼尼龙均可有效调节博来霉素诱导的大鼠肺纤维化进程。
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