Ling J M, Char T S, Cheng A F
Department of Microbiology, The Chinese University of Hong Kong, The Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR, China.
J Infect. 2002 Nov;45(4):257-62. doi: 10.1053/jinf.2002.1054.
To study the distribution of hospital isolates of enterococci from urines, bile, blood and body fluids and to evaluate different methods for the identification of enterococci.
Enterococci isolated from urine, bile, blood and body fluids collected during 1997 and 1998 were identified by polymerase chain reaction (PCR), API 20 Strep and conventional biochemical tests.
A total of 498 non-duplicate enterococci were studied: 398 and 43 isolates from urine and bile, respectively, 49 from blood, two from cerebrospinal fluid and six from body fluids. Both API 20 Strep and PCR gave the same identification results for 240 Enterococcus faecalis isolates, 45 E. faecium isolates and one isolate each of E. gallinarum and E. Casseliflavus. These isolates were re-defined by conventional biochemical tests. PCR could correctly identify 303 (98%) isolates while API 20 Strep could only correctly identify 287 (93%) isolates (99% of E. faecalis and 57-87% of the other Enterococcus sp.). Thus, PCR was used in the identification of the remaining isolates and the identity of isolates other than E. faecalis was subsequently confirmed by biochemical tests.
The majority of enterococci isolated was E. faecalis (81%) while only 15% were E. faecium and 4% the other enterococcal species. PCR could correctly identify E. faecalis while the identity of other enterococcal species had to be confirmed by biochemical tests.
研究从尿液、胆汁、血液和体液中分离出的医院内肠球菌的分布情况，并评估鉴定肠球菌的不同方法。
对1997年和1998年期间从尿液、胆汁、血液和体液中分离出的肠球菌，采用聚合酶链反应（PCR）、API 20 Strep和传统生化试验进行鉴定。
共研究了498株非重复肠球菌：分别从尿液和胆汁中分离出398株和43株，从血液中分离出49株，从脑脊液中分离出2株，从体液中分离出6株。对于240株粪肠球菌、45株屎肠球菌、1株鹑鸡肠球菌和1株格氏肠球菌，API 20 Strep和PCR给出了相同的鉴定结果。这些分离株通过传统生化试验重新定义。PCR能正确鉴定303株（98%）分离株，而API 20 Strep只能正确鉴定287株（93%）分离株（粪肠球菌的99%，其他肠球菌属的57 - 87%）。因此，PCR用于鉴定其余分离株，除粪肠球菌外的分离株的身份随后通过生化试验得以确认。
分离出的肠球菌大多数为粪肠球菌（81%），而屎肠球菌仅占15%，其他肠球菌属占4%。PCR能正确鉴定粪肠球菌，而其他肠球菌属的身份必须通过生化试验来确认。
