Hoffmann Friederike, Rapp Hans Tore, Zöller Tobias, Reitner Joachim
University of Göttingen, Department of Geosciences, Geobiology Group, Goldschmidtstr. 3, 37077 Göttingen, Germany.
J Biotechnol. 2003 Jan 23;100(2):109-18. doi: 10.1016/s0168-1656(02)00258-4.
A cultivation method has been developed for the boreal deep-water sponge Geodia barretti (Demospongiae, Geodiidae), a species which is common in the deep Norwegian fjords. The species is known to contain secondary metabolites which are biologically active. Choanosomal fragments of 2-4 cm(3) (approximately 3-7 g) were kept in half-open systems. Cicatrisation and regeneration processes were surveyed by histological examination during 8 months of cultivation. During the first weeks, the weight of the fragments decreased. However, after about 6 weeks the weight equalled the original weight, and after 1 year the weight had increased by about 40% compared to the original weight. The initial decrease was due to complex healing processes and the regeneration of the cortex, a sterrastral layer typical for the family of the Geodiidae. We document, for the first time, the complete cortex reconstruction in an adult G. barretti, as well as the development of egg cells during cultivation. Our study represents the first attempt at biotechnological production of boreal sponge tissue. For successful farming of G. barretti and other boreal and arctic sponges, however, further investigation is needed on factors stimulating growth and secondary metabolite production in the target species.
已经开发出一种针对北方深水海绵Geodia barretti（寻常海绵纲，地海绵科）的养殖方法，该物种在挪威深水峡湾很常见。已知该物种含有具有生物活性的次生代谢产物。将2 - 4立方厘米（约3 - 7克）的中胶层碎片置于半开放系统中。在8个月的养殖期间，通过组织学检查对愈合和再生过程进行了监测。在最初的几周里，碎片的重量下降。然而，大约6周后重量恢复到原始重量，1年后重量相比原始重量增加了约40%。最初的重量下降是由于复杂的愈合过程以及皮层的再生，皮层是地海绵科特有的一层骨针层。我们首次记录了成年巴氏地海绵皮层的完全重建以及养殖过程中卵细胞的发育。我们的研究代表了北方海绵组织生物技术生产的首次尝试。然而，为了成功养殖巴氏地海绵以及其他北方和北极海绵，需要进一步研究刺激目标物种生长和次生代谢产物产生的因素。
