在生物反应器中对海洋海绵安德鲁氏拟软海绵进行可控培养的进展。

Progress towards a controlled culture of the marine sponge Pseudosuberites andrewsi in a bioreactor.

作者信息

Osinga Ronald, Belarbi El Hassan, Grima Emilio Molina, Tramper Johannes, Wijffels René H

机构信息

Food and Bioprocess Engineering Group, Department of Food Science and Technology, Wageningen Agricultural University, PO Box 8129, 6700 EV Wageningen, The Netherlands.

出版信息

J Biotechnol. 2003 Jan 23;100(2):141-6. doi: 10.1016/s0168-1656(02)00257-2.

DOI:10.1016/s0168-1656(02)00257-2

PMID:12423908

Abstract

Explants of the tropical sponge Pseudosuberites andrewsi were fed with the marine diatom Phaeodactylum tricornotum. The food was supplied either as intact algae or as a filtered crude extract. Growth (measured as an increase in underwater weight) was found in both experiments. The explants fed with intact algae increased to an average underwater weight of 255% of the initial weight in 45-60 days. The explants fed with crude extract increased to an average of 200% of the initial weight in 30 days. These results show that it is possible to grow a sponge using a single microorganism species as a food source. In addition, it was demonstrated that sponges are also capable of growing on non-particulate food. Therefore, this study is an important step forward towards the development of controlled, in vivo sponge cultures.

摘要

用海洋硅藻三角褐指藻喂养热带海绵安德鲁伪软海绵的外植体。食物以完整藻类或过滤后的粗提物形式提供。在两个实验中均发现了生长情况（以水下重量增加来衡量）。用完整藻类喂养的外植体在45 - 60天内平均水下重量增加到初始重量的255%。用粗提物喂养的外植体在30天内平均增加到初始重量的200%。这些结果表明，有可能使用单一微生物物种作为食物来源来培育海绵。此外，还证明了海绵也能够以非颗粒性食物生长。因此，这项研究是朝着开发可控的体内海绵培养迈出的重要一步。