Alzoreky N S, Nakahara K
Faculty of Agriculture, Department of Food Science, Sana'a University, Sana'a, Yemen.
Int J Food Microbiol. 2003 Feb 15;80(3):223-30. doi: 10.1016/s0168-1605(02)00169-1.
Extracts of edible plants (26 species) from China, Japan, Thailand and Yemen were screened for their antibacterial activity against Bacillus cereus, Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salmonella infantis. Buffered methanol (80% methanol and 20% PBS) and acetone extracted inhibitory substances against tested bacteria from 16 plants, as revealed by the disc assay. The minimum inhibitory concentrations (MICs) of extracts determined by the agar dilution method ranged from 165 to 2640 mg l(-1). The most sensitive microorganism to extracts from Azadirachta indica, Cinnamomum cassia, Rumex nervosus, Ruta graveolens, Thymus serpyllum and Zingiber officinale was B. cereus, with MIC of 165 to 660 mg l(-1). E. coli and S. infantis were only inhibited by Cinnamomum cassia extracts at the highest MIC (2640 mg l(-1)). L. monocytogenes (Tottori) was more resistant than the ATCC 7644 strain to extracts from Ruta chalepensis, Artemisia absinthium and Cissus spp. EDTA (0.85 mM) reduced the MICs of Cinnamomum cassia and Cissus rotundifolia by at least 50% when tested against E. coli, S. infantis, S. aureus and L. monocytogenes.
对来自中国、日本、泰国和也门的可食用植物（26种）提取物进行了筛选，检测其对蜡样芽孢杆菌、金黄色葡萄球菌、单核细胞增生李斯特菌、大肠杆菌和婴儿沙门氏菌的抗菌活性。纸片扩散法显示，缓冲甲醇（80%甲醇和20%磷酸盐缓冲液）和丙酮从16种植物中提取出了对受试细菌有抑制作用的物质。用琼脂稀释法测定的提取物最低抑菌浓度（MIC）范围为165至2640 mg l(-1)。对印楝、肉桂、皱叶酸模、芸香、百里香和姜的提取物最敏感的微生物是蜡样芽孢杆菌，MIC为165至660 mg l(-1)。大肠杆菌和婴儿沙门氏菌仅在最高MIC（2640 mg l(-1)）时受到肉桂提取物的抑制。单核细胞增生李斯特菌（鸟取株）比ATCC 7644菌株对药用芸香、苦艾和白粉藤提取物更具抗性。当针对大肠杆菌、婴儿沙门氏菌、金黄色葡萄球菌和单核细胞增生李斯特菌进行测试时，0.85 mM的乙二胺四乙酸（EDTA）使肉桂和圆叶白粉藤的MIC至少降低了50%。
