Alamry Khalid A, Hussein Mahmoud A, Khan Ajahar, Asiri Abdullah M
Faculty of Science, Department of Chemistry, King Abdulaziz University Jeddah 21589 Saudi Arabia
Chemistry Department, Faculty of Science, Assiut University Assiut 71516 Egypt.
RSC Adv. 2024 Jul 12;14(30):22017-22027. doi: 10.1039/d4ra02893g. eCollection 2024 Jul 5.
Polymeric bio-composites synthesized a green approach using natural herbs have fascinating anticoagulant activity due to their eco-friendly and non-toxic behavior towards various physical and chemical actions. Herein, we introduce a simple and eco-friendly approach for the fabrication of a new hybrid type of bio-composite based on sulfated carboxymethyl cellulose (S-CMC) and leaf powder (S-CMC/NLP). First, a non-toxic sulfating agent called N(SONa) was used to modify carboxymethyl cellulose into S-CMC. With an ion exchange capacity of 0.25 meq. g, the level of sulfation (%) of S-CMC (modified polysaccharide) was measured to be 12.01%. Three types of S-CMC/NLP bio-composites were developed by varying the concentration of NLP. FE-SEM, EDX, and XRD were used to characterize the structural features of S-CMC/NLP bio-composites. FTIR spectroscopy indicated that the S-CMC/NLP bio-composite possesses COO, -OH and SO groups, suggesting the structural similarity to heparin. In addition, the anticoagulant effect of the S-CMC/NLP bio-composite was investigated using PT and APTT assays. The APTT investigation confirmed that following the intrinsic pathway of the coagulation system, 2-NLP/S-CMC bio-composite dose-dependently (0.045-0.28 mg mL) prolonged the time of blood coagulation compared to control (pure plasma). The S-CMC/NLP bio-composite showed its potential as a new, safe, and effective candidate for anticoagulant activity.
采用天然草药通过绿色方法合成的聚合物生物复合材料，因其对各种物理和化学作用具有生态友好且无毒的特性，而具有迷人的抗凝血活性。在此，我们介绍一种简单且生态友好的方法，用于制备基于硫酸化羧甲基纤维素（S-CMC）和叶粉（S-CMC/NLP）的新型杂化生物复合材料。首先，使用一种名为N(SONa)的无毒硫酸化剂将羧甲基纤维素改性为S-CMC。S-CMC（改性多糖）的硫酸化程度（%）经测量为12.01%，离子交换容量为0.25 meq. g。通过改变NLP的浓度，制备了三种类型的S-CMC/NLP生物复合材料。使用场发射扫描电子显微镜（FE-SEM）、能量散射X射线谱（EDX）和X射线衍射（XRD）对S-CMC/NLP生物复合材料的结构特征进行表征。傅里叶变换红外光谱（FTIR）表明，S-CMC/NLP生物复合材料具有COO、-OH和SO基团，表明其结构与肝素相似。此外，使用凝血酶原时间（PT）和活化部分凝血活酶时间（APTT）测定法研究了S-CMC/NLP生物复合材料的抗凝血作用。APTT研究证实，遵循凝血系统的内源性途径，与对照（纯血浆）相比，2-NLP/S-CMC生物复合材料以剂量依赖方式（0.045 - 0.28 mg mL）延长了血液凝固时间。S-CMC/NLP生物复合材料显示出作为一种新型、安全且有效的抗凝血活性候选物的潜力。
