Cobo Eduardo Rubén, Cano Dora, Rossetti Osvaldo, Campero Carlos Manuel
Consejo Nacional de Investigaciones Cienti;ficas y Técnicas (CONICET), Argentina.
Vet Parasitol. 2002 Nov 11;109(3-4):169-84. doi: 10.1016/s0304-4017(02)00301-1.
The performance of a whole-cell vaccine and the other vaccine with cellular membranes of Tritrichomonas foetus applied to heifers naturally challenged by mating with an infected bull was determined. Forty heifers were divided into three groups: a control group (n=16) without immunizing, another group (n=12) immunized with whole cells (10(8)/dose) and a third group (n=12) immunized with cellular membranes (300 micro g of membranes/dose protein). The females were subcutaneously vaccinated at 3-week on two occasions and received a third intravaginal booster dose. After 3 weeks of the last vaccinal doses, the heifers were served by a T. foetus infected bull over 90-day period. The mean duration of infection for membrane-vaccinated heifers was 60 days +/-25, compared with 63 days +/-35.8 of infection for whole-cell-vaccinated heifers and 79 days +/-41.3 for control heifers. Calving rates were 6/12 for membrane-vaccinated heifers, 3/12 for whole-cell-vaccinated animals, and 2/16 for control animals. Fetal mortality rates were 3/12 for membrane-vaccinated animals, 4/12 for those vaccinated with whole cells and 10/16 for control animals. These reproductive parameters were significantly different (P<0.05) between heifers vaccinated with membranes and control heifers. The hemolytic test and enzyme-linked immunoabsorbent assay (ELISA) with T. foetus antigen showed that serum immunoglobulins peaked before and during the breeding period. The heifers vaccinated with membranes developed an important response during the critical period of fetal loss, second and third month of the breeding time, and another month after the same period. The ELISA method was more sensitive and more reliable than the hemolytic test for the evaluation of the systemic immune response in females infected and/or vaccinated with T. foetus.
对一种全细胞疫苗以及另一种含有胎儿三毛滴虫细胞膜的疫苗在与感染公牛交配而自然受到感染的小母牛身上的效果进行了测定。40头小母牛被分为三组：一组为未免疫的对照组（n = 16），另一组（n = 12）用全细胞（10⁸/剂量）免疫，第三组（n = 12）用细胞膜（300μg膜/剂量蛋白）免疫。这些雌性动物在3周龄时皮下接种疫苗，共接种两次，并接受第三次阴道内加强剂量。在最后一次接种疫苗3周后，小母牛与感染胎儿三毛滴虫的公牛交配90天。接种细胞膜疫苗的小母牛的平均感染持续时间为60天±25天，接种全细胞疫苗的小母牛为63天±35.8天，对照组小母牛为79天±41.3天。接种细胞膜疫苗的小母牛的产犊率为6/12，接种全细胞疫苗的动物为3/12，对照组动物为2/16。接种细胞膜疫苗的动物的胎儿死亡率为3/12，接种全细胞疫苗的动物为4/12，对照组动物为10/16。接种细胞膜疫苗的小母牛与对照小母牛之间的这些繁殖参数有显著差异（P<0.05）。用胎儿三毛滴虫抗原进行的溶血试验和酶联免疫吸附测定（ELISA）表明，血清免疫球蛋白在繁殖期之前和期间达到峰值。接种细胞膜疫苗的小母牛在繁殖期第二个和第三个月以及该时期之后的另一个月胎儿丢失的关键时期产生了重要反应。对于评估感染和/或接种胎儿三毛滴虫的雌性动物的全身免疫反应，ELISA方法比溶血试验更敏感、更可靠。
