Campero C M, Hirst R G, Ladds P W, Vaughan J A, Emery D L, Watson D L
Graduate School of Tropical Veterinary Science, James Cook University of North Queensland, Townsville.
Aust Vet J. 1990 May;67(5):175-8. doi: 10.1111/j.1751-0813.1990.tb07749.x.
An enzyme-linked immunosorbent assay (ELISA) was developed for detecting antibody to Tritrichomonas foetus using both whole cell antigen (WCA) and membrane protein antigen (MPA). The test was used to detect specific antibody in serum, preputial washings and seminal plasma samples from 7 adult bulls which were vaccinated subcutaneously on 3 occasions with a membrane protein vaccine against T. foetus var brisbane in an oil adjuvant, and from 4 unvaccinated control animals. One month after administration of the third dose of vaccine, vaccinated and control bulls were repeatedly challenged with the live vaccine strain of the T. foetus. A steady increase in serum antibody titre was detected after each inoculation of vaccine when both antigens were used in the ELISA. However, MPA was more sensitive. After challenge, vaccinated bulls developed an increased titre. No specific antibody was detected in control bulls, except in one bull after challenge in which seroconversion was detected. The serum antibody titres of both groups of animals were also measured with the microagglutination test which proved less sensitive than the ELISA. Antibody titres to both antigens, although lower than in serum, were detected in the seminal plasma of vaccinated animals. The control bulls remained non-responsive. No antibody was detected by ELISA in preputial washings from either control or vaccinated bulls prior to challenge. Post-challenge, some of the vaccinated bulls were responsive with both antigens whereas the control bulls remained negative.
开发了一种酶联免疫吸附测定（ELISA），用于使用全细胞抗原（WCA）和膜蛋白抗原（MPA）检测胎儿三毛滴虫抗体。该试验用于检测7头成年公牛血清、包皮冲洗液和精浆样本中的特异性抗体，这些公牛分3次皮下接种了含油佐剂的针对布里斯班胎儿三毛滴虫变种的膜蛋白疫苗，还检测了4头未接种疫苗的对照动物。在接种第三剂疫苗一个月后，给接种疫苗的公牛和对照公牛反复接种胎儿三毛滴虫活疫苗株。当ELISA中使用两种抗原时，每次接种疫苗后血清抗体滴度均稳步上升。然而，MPA更敏感。接种疫苗的公牛在攻毒后滴度升高。对照公牛中未检测到特异性抗体，只有一头公牛在攻毒后出现了血清学转换。还用微凝集试验测量了两组动物的血清抗体滴度，结果证明该试验不如ELISA敏感。在接种疫苗动物的精浆中检测到了针对两种抗原的抗体滴度，尽管低于血清中的滴度。对照公牛仍无反应。在攻毒前，对照公牛和接种疫苗公牛的包皮冲洗液中均未通过ELISA检测到抗体。攻毒后，一些接种疫苗的公牛对两种抗原均有反应，而对照公牛仍为阴性。
