Han In Suk, Han Man-Hee, Kim Jinwon, Lew Seok, Lee Young Jun, Horkay Ferenc, Magda Jules J
M-Biotech Inc., 2411 South 1070 West, Suite C, Salt Lake City, Utah 84119, USA.
Biomacromolecules. 2002 Nov-Dec;3(6):1271-5. doi: 10.1021/bm0255894.
A new type of biosensor is proposed that combines the recognition properties of "intelligent" hydrogels with the sensitivity and reliability of microfabricated pressure transducers. In the proposed device, analyte-induced changes in the osmotic swelling pressure of an environmentally responsive hydrogel are measured by confining it within a small implantable enclosure between a rigid semipermeable membrane and the diaphragm of a miniature pressure transducer. Proof-of-principle tests of this device were performed in vitro using pH-sensitive hydrogels, with osmotic deswelling data for the same hydrogels used as a benchmark for comparison. The swelling pressure of the hydrogel was accurately determined from osmotic deswelling measurements against reservoirs of known osmotic stress. Values of swelling pressure vs salt concentration measured with a preliminary version of the sensor agree well with osmotic deswelling results. Through modification of the hydrogel with various enzymes or pendant binding moieties, the sensor has the potential to detect a wide range of biological analytes with good specificity.
提出了一种新型生物传感器，它将“智能”水凝胶的识别特性与微制造压力传感器的灵敏度和可靠性相结合。在所提出的装置中，通过将环境响应性水凝胶限制在刚性半透膜和微型压力传感器的隔膜之间的小型可植入外壳内，来测量分析物引起的水凝胶渗透膨胀压力的变化。使用pH敏感水凝胶在体外对该装置进行了原理验证测试，并将相同水凝胶的渗透去膨胀数据用作比较基准。通过针对已知渗透应力的储液器进行渗透去膨胀测量，准确确定了水凝胶的膨胀压力。用该传感器的初步版本测量的膨胀压力与盐浓度的值与渗透去膨胀结果非常吻合。通过用各种酶或侧链结合部分修饰水凝胶，该传感器有可能以良好的特异性检测广泛的生物分析物。
