Runnebaum B, Runnebaum H, Stöber I, Zander J
Acta Endocrinol (Copenh). 1975 Nov;80(3):558-68. doi: 10.1530/acta.0.0800558.
The endogenous levels of progesterone, 20 alpha-dihydroprogesterone and 20 beta-dihydroprogesterone in different foetal tissues, in placental tissue and in foetal blood during pregnancy (week 9-25) were determined by gas-liquid chromatography or by radioimmunoassay. The identification of these steroids in foetal tissues was based on the behaviour in paper and thin-layer chromatography, the formation of different derivatives, and the retention times in gas-liquid chromatography. For additional identification of progesterone and 20 alpha-dihydroprogesterone a mass spectrum was obtained. The average concentrations of progesterone (P), 20 alpha-dihydroprogesterone (20 alpha-DHP) and 20 beta-dihydroprogesterone (20 beta-DHP) in foetal tissues were as follows (ng/g wet weight tissue): brain, P 119.0 +/- 45.5, 20 alpha-DHP 86.9 +/- 44.7 and 20 beta-DHP 9.7; lungs, P 257.7 +/- 195.7, 20 alpha-DHP 60.3 +/- 26.4 and 20 beta-DHP 7.8; liver, P 103.7 +/- 84.6, 20 alpha-DHP 95.2 +/- 89.8 and 20 beta-DHP 17.8; adrenals, P 295.7 +/- 90.0 20 alpha-DHP 254.5 +/- 194.6; kidneys, P 214.2 +/- 155.3 and 20 alpha-DHP 76.6 +/- 43.0; intestine, P 273.2 +/- 166.7, 20 alpha-DHP 77.9 +/- 51.2 and 20 beta-DHP 8.7; residual tissues, P 246.3 +/- 178.3 and 20 alpha-DHP 54.8 +/- 44.5. The average concentrations in placental tissue and in foetal plasma (week 12-18) were as follows (ng/g wet weight tissue or ng/ml plasma): placenta, P 2619. +/- 2153.6 and 20 alpha-DHP 461.0 +/- 155.9; foetal plasma, P 378.7 +/- 231.8 and 20 alpha-DHP 283.0 +/- 108.9. These results indicate that some foetal organs contain a high 20 alpha-reductase activity at least during the first half of pregnancy.
采用气液色谱法或放射免疫分析法测定了孕期（第9 - 25周）不同胎儿组织、胎盘组织及胎儿血液中孕酮、20α-二氢孕酮和20β-二氢孕酮的内源性水平。胎儿组织中这些类固醇的鉴定基于纸色谱和薄层色谱行为、不同衍生物的形成以及气液色谱中的保留时间。为进一步鉴定孕酮和20α-二氢孕酮，获得了质谱图。胎儿组织中孕酮（P）、20α-二氢孕酮（20α-DHP）和20β-二氢孕酮（20β-DHP）的平均浓度如下（ng/g湿重组织）：脑，P 119.0±45.5，20α-DHP 86.9±44.7，20β-DHP 9.7；肺，P 257.7±195.7，20α-DHP 60.3±26.4，20β-DHP 7.8；肝，P 103.7±84.6，20α-DHP 95.2±89.8，20β-DHP 17.8；肾上腺，P 295.7±90.0，20α-DHP 254.5±194.6；肾，P 214.2±155.3，20α-DHP 76.6±43.0；肠，P 273.2±166.7，20α-DHP 77.9±51.2，20β-DHP 8.7；残余组织，P 246.3±178.3，20α-DHP 54.8±44.5。胎盘组织和胎儿血浆（第12 - 18周）中的平均浓度如下（ng/g湿重组织或ng/ml血浆）：胎盘，P 2619.±2153.6，20α-DHP 461.0±155.9；胎儿血浆，P 378.7±231.8，20α-DHP 283.0±108.9。这些结果表明，至少在孕期的前半段，一些胎儿器官具有较高的20α-还原酶活性。
