Fernandez F, Hinton M, Van Gils B
Division of Food Animal Science, Department of Clinical Veterinary Science, University of Bristol, Langford, Avon BS40 5DU, UK.
Avian Pathol. 2002 Feb;31(1):49-58. doi: 10.1080/03079450120106000.
This study first investigates the effects of mash diet, or mash supplemented with either 2.5% mannose-oligosaccharide (MOS) or palm kernel meal (PKM), on the microflora of the hen caecal contents. Second, it investigates the effect of caecal contents of hens (HCC) fed mash or mash supplemented with MOS or PKM on the major microflora groups of chicks, and their inhibitory effect on Salmonella enterica serovar Enteritidis (PT4) colonization. Finally, this study investigates the effect over time of diets supplemented with MOS or PKM on S. Enteritidis colonization and the microflora of chicks. In hens, supplemented diets increased Bifidobacterium spp., while decreasing members of Enterobacteriaceae and Enterococcus spp., compared with the mash diet. Chicks dosed with the HCC showed, on average, increased numbers of anaerobes, while the numbers of aerobes decreased including coliforms and S. Enteritidis compared with controls without HCC. In chicks fed the MOS-supplemented or PKM-supplemented diets, S. Enteritidis colonization decreased over time, compared with mash alone. Four-week-old PKM birds showed an increase in Bifidobacterium spp. and Lactobacillus spp., with a decrease in S. Enteritidis compared with week 2. Generally, the HCC and diets supplemented with MOS or PKM affected the birds intestinal microflora by increasing the Bifidobacterium spp. and Lactobacillus spp., while decreasing the Enterobacteriaceae groups. They also reduced susceptibility in young chickens to colonization by S. Enteritidis.
本研究首先调查了粉料日粮，或添加2.5%甘露寡糖（MOS）或棕榈仁粕（PKM）的粉料日粮对母鸡盲肠内容物微生物区系的影响。其次，研究了饲喂粉料或添加MOS或PKM的粉料日粮的母鸡盲肠内容物（HCC）对雏鸡主要微生物菌群的影响，以及它们对肠炎沙门氏菌肠炎血清型（PT4）定植的抑制作用。最后，本研究调查了添加MOS或PKM的日粮随时间对肠炎沙门氏菌定植和雏鸡微生物区系的影响。与粉料日粮相比，在母鸡中，添加日粮增加了双歧杆菌属的数量，同时减少了肠杆菌科和肠球菌属的成员。与未接种HCC的对照组相比，接种HCC的雏鸡平均厌氧菌数量增加，而需氧菌数量减少，包括大肠菌群和肠炎沙门氏菌。与仅饲喂粉料相比，饲喂添加MOS或PKM日粮的雏鸡，肠炎沙门氏菌的定植随时间减少。与第2周相比，4周龄饲喂PKM的雏鸡双歧杆菌属和乳酸杆菌属数量增加，肠炎沙门氏菌数量减少。总体而言，HCC以及添加MOS或PKM的日粮通过增加双歧杆菌属和乳酸杆菌属数量，同时减少肠杆菌科菌群，影响了家禽的肠道微生物区系。它们还降低了雏鸡对肠炎沙门氏菌定植的易感性。
